一种用于检测细胞表面抗原的膜通透性试验。
A membrane permeability test for the detection of cell surface antigens.
作者信息
Kurth R, Medley G
出版信息
Immunology. 1975 Nov;29(5):803-11.
Abstract
A convenient microtest is described which utilizes antibody-mediated release of [14C]nicotinamide ([14C]NA) from target cells for the detection of cell surface antigens. This test is considerably more sensitive and faster than the widely used 51Cr release test because most of the [14C]NA is rapidly released from the target cells in the initial phase of membrane permeability changes induced by activated complement, as distinct from the colloid osmotic phase of complement-mediated cytolysis.
摘要
本文描述了一种简便的微量试验，该试验利用抗体介导的从靶细胞中释放[14C]烟酰胺（[14C]NA）来检测细胞表面抗原。该试验比广泛使用的51Cr释放试验更为灵敏且快速，因为在活化补体诱导的膜通透性变化的初始阶段，大部分[14C]NA会迅速从靶细胞中释放出来，这与补体介导的细胞溶解的胶体渗透阶段不同。
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