单层细胞表面抗原的检测。I. 微量免疫黏附的应用。
Detection of cell surface antigens on monolayer cells. I. The application of immune adherence on a micro scale.
作者信息
Tachibana T, Klein E
出版信息
Immunology. 1970 Nov;19(5):771-82.
Abstract
A small scale modified method of the immune adherence on monolayer cells grown in Falcon plastic `Microtest' plates is described. This method is especially useful in mouse systems as it simplifies the washing procedure which is an indispensable step for removal of the anticomplementary activity present in mouse serum. Histocompatibility antigens and the surface antigen induced by the Moloney virus could be detected with this method.
摘要
本文描述了一种在Falcon塑料“微量检测”板中生长的单层细胞上进行免疫黏附的小规模改良方法。该方法在小鼠系统中特别有用，因为它简化了洗涤程序，而洗涤程序是去除小鼠血清中存在的抗补体活性必不可少的步骤。用这种方法可以检测组织相容性抗原和莫洛尼病毒诱导的表面抗原。
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