Furda J, Morgenstern S, Snyder L R
Clin Chem. 1976 Jul;22(7):1042-6.
The Jendrassik--Groff assay for direct bilirubin was adapted for analysis rates of 150/h on the Technicon "SMAC" continuous-flow analyzer. This requires development of a standard that is compatible with the other 19 channels on this analyzer. N-1-Naphthyl ethylenediamine dihydrochloride has been used for standardization of direct bilirubin assays, but we found it to be unsuitable because values for potassium are falsely elevated when potassium is determined with a valinomycin ion-selective electrode. This interference can be eliminated by alkylating the aliphatic amine group in the standard. The resulting compounds undergo the coupling reaction in the same way as the original compound and function equally well as standards for the direct bilirubin reaction. The only limitation of these analogs is their decreased solubility at physiological pH in some cases. Thus only certain alkyl groups are suitable.
将用于直接胆红素检测的简氏（Jendrassik）-格罗夫（Groff）检测法进行了调整，以便在Technicon “SMAC” 连续流动分析仪上实现每小时150次的分析速度。这需要开发一种与该分析仪上其他19个通道兼容的标准品。N-1-萘基乙二胺二盐酸盐已被用于直接胆红素检测的标准化，但我们发现它并不合适，因为在用缬氨霉素离子选择性电极测定钾时，钾的值会被错误地升高。这种干扰可以通过将标准品中的脂肪胺基团烷基化来消除。生成的化合物与原始化合物以相同的方式进行偶联反应，并且作为直接胆红素反应的标准品同样有效。这些类似物的唯一局限性是在某些情况下它们在生理pH值下的溶解度降低。因此只有某些烷基是合适的。
