Hadiani M R, Yazdanpanah H, Ghazi-Khansari M, Cheraghali A M, Goodarzi M
Department of Pharmacology, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran.
Food Addit Contam. 2003 Apr;20(4):380-5. doi: 10.1080/0265203031000087968.
During September 2000, forty samples of preharvest maize from the province of Mazandaran, north Iran, were randomly collected. Samples were analysed for zearalenone (ZEA) by a thin-layer chromatography (TLC) method (AOAC Official Method). ZEA was extracted with chloroform, purified through a chromatographic column containing silica gel, separated on a TLC plate and quantified by densitometry. The analytical method was validated and was adequately reliable and sensitive. The mean recovery rate of ZEA from spiked samples was 92%. The absolute amount of ZEA standard detectable on a TLC plate was 20 ng, giving a limit of detection (LOD) of 100 ng g(-1). In some samples, it was shown that aflatoxins interfere with ZEA. Therefore, to remove this interference, the TLC mobile phase was changed. Data revealed that three of 40 (7.5%) maize samples contained ZEA in the range 100-212 ng g(-1), with a mean of 141+/-51 ng g(-1). This study, which is the first report of ZEA occurrence in Iranian maize, showed that the ZEA level in maize of Mazandaran province was lower than maximum limit for this mycotoxin in Iran.
2000年9月期间，从伊朗北部马赞德兰省随机采集了40份收获前玉米样本。采用薄层色谱法（AOAC官方方法）分析样本中的玉米赤霉烯酮（ZEA）。用氯仿提取ZEA，通过装有硅胶的色谱柱进行纯化，在薄层板上分离并用密度测定法定量。该分析方法经过验证，具有足够的可靠性和灵敏度。加标样本中ZEA的平均回收率为92%。薄层板上可检测到的ZEA标准品绝对量为20 ng，检测限（LOD）为100 ng g⁻¹。在一些样本中，黄曲霉毒素干扰了ZEA的检测。因此，为消除这种干扰，改变了薄层色谱流动相。数据显示，40份玉米样本中有3份（7.5%）含有ZEA，含量在100 - 212 ng g⁻¹之间，平均为141 ± 51 ng g⁻¹。本研究是伊朗玉米中ZEA存在情况的首次报道，表明马赞德兰省玉米中的ZEA水平低于伊朗该霉菌毒素的最大限量。
