[红车轴草提取物中异黄酮测定方法的研究]
[Study of the methods of determining the isoflavones in herbs of Trifolium pratense extractive].
作者信息
Bian Qing-quan, Yang Zhen-ping, Ma Mei-zhong, Li Song
机构信息
Mianyang Teacher's College, Mianyang 621000, Sichuan, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2003 Nov;28(11):1044-7.
OBJECTIVE
To study the methods of determining the amount of Isoflavones in red clover extractive.
METHOD
RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm.
RESULT
After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%.
CONCLUSION
A simple, reliable and effective quality-control method is given.
目的
研究红三叶草提取物中异黄酮含量的测定方法。
方法
采用反相高效液相色谱法测定异黄酮，使用C18反相柱，流动相为甲醇-乙腈-0.1%磷酸，检测波长为260nm。
结果
4种黄酮类成分在水溶后得到满意分离，进样量在0.024～0.336μg范围内与峰面积有良好的线性关系，总异黄酮测定结果重复性好，RSD=10.1%。
结论
给出了一种简便、可靠、有效的质量控制方法。
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