Tian Zeng-Yuan, Dai Jing-Rui
National Maize Improvement Center of China, China Agricultural University, Beijing 100094, China.
Yi Chuan Xue Bao. 2003 Feb;30(2):154-62.
To understand the molecular mechanism of maize heterosis, differential gene expression patterns in functional leaves between 10 maize inbreds and 38 hybrids at spikelet differentiation stage were analyzed by using cDNA-AFLP. The correlation analysis of various differential gene expression patterns with the performance and heterosis of main maize agronomic traits was carried out. The main results are as follows: (1) There are differential gene expression patterns in quality and quantity between hybrids and their parents. The differential expression patterns in quality include: bands expressed only in one parent, bands expressed only in both parents, bands expressed only in one parent and F1, bands expressed only in F1. (2) At spikelet differentiation stage, there are large variations among different hybrids for the same differentially expressed patterns. In general from mean data, there were 25.22% bands expressed only in F1, 21.46% bands expressed in one parent and F1, 8.27% bands expressed only in both parents and 33.49% bands expressed only in one parent. (3) For bands expressed only in one parent, significant positive correlation was detected with the relationship to the performance of plant height. For bands expressed only in both parents, significant negative correlation was detected with the relationship to the heterosis of ear diameter. For bands expressed only in one parent and F1, significant negative correlations were detected with the relationships to the heterosis of rows per ear and seed weight per ear. However, for bands expressed only in F1, and for bands only in two parents or only in F1, no significant correlation was detected with the relationship to the performance and heterosis of all agronomic traits.
为了解玉米杂种优势的分子机制，利用cDNA-AFLP技术分析了10个玉米自交系和38个杂交种在小穗分化期功能叶中的差异基因表达模式。对各种差异基因表达模式与玉米主要农艺性状的表现及杂种优势进行了相关性分析。主要结果如下：（1）杂交种与其亲本在质量和数量上存在差异基因表达模式。质量上的差异表达模式包括：仅在一个亲本中表达的条带、仅在双亲中表达的条带、仅在一个亲本和F1中表达的条带、仅在F1中表达的条带。（2）在小穗分化期，同一差异表达模式在不同杂交种间存在较大差异。从平均数据来看，一般有25.22%的条带仅在F1中表达，21.46%的条带在一个亲本和F1中表达，8.27%的条带仅在双亲中表达，33.49%的条带仅在一个亲本中表达。（3）对于仅在一个亲本中表达的条带，与株高表现呈显著正相关。对于仅在双亲中表达的条带，与穗粗杂种优势呈显著负相关。对于仅在一个亲本和F1中表达的条带与每行粒数和每穗粒重杂种优势呈显著负相关。然而，对于仅在F1中表达的条带以及仅在双亲或仅在F1中表达的条带，与所有农艺性状的表现及杂种优势均未检测到显著相关性。
