Xing Chao-Zhu, Zhao Yun-Lei, Yu Shu-Xun, Zhang Xian-Long, Guo Li-Ping, Wang Hai-Lin
Cotton Research Institute, Chinese Academy of Agriculture Sciences, Anyang, China.
Yi Chuan Xue Bao. 2006 Oct;33(10):948-56. doi: 10.1016/S0379-4172(06)60129-7.
cDNAs of leaves of 24 pest-resistant cotton (authorization) crosses and their parents in full opening flower stages were amplified and different bands were displayed by DDRT-PCR, with 45 primer combinations. The mean percentage of bands duplicated was 70.1%, which implied that there was a high proportion of pseudopositive fragments in the amplified cDNA. These pseudopositive bands can be reduced using duplicate PCR. Correlation analysis between differential gene expression and hybrid performance and heterosis showed that M4 (bands detected in one parent and F1) was positively correlated with all yield traits, and significantly correlated with boll number; M2 (bands observed in one parent but not in F1 and another parent) was negatively correlated with heterosis of boll number; and M3 (bands observed only in F1) was positively correlated with heterosis of lint yield. These results showed that genes that had dominant and specific expression in top leaves of full opening flower stages were beneficial to yield formation and heterosis occurrence.
对24个抗虫棉（审定）杂交组合及其亲本在盛花期叶片的cDNA进行扩增，采用45对引物组合通过DDRT-PCR显示出不同条带。扩增cDNA中条带重复出现的平均比例为70.1%，这表明扩增的cDNA中假阳性片段比例较高。使用重复PCR可减少这些假阳性条带。差异基因表达与杂种表现和杂种优势的相关性分析表明，M4（在一个亲本和F1中检测到的条带）与所有产量性状呈正相关，与铃数显著相关；M2（在一个亲本中观察到但在F1和另一个亲本中未观察到的条带）与铃数杂种优势呈负相关；M3（仅在F1中观察到的条带）与皮棉产量杂种优势呈正相关。这些结果表明，在盛花期顶部叶片中具有显性和特异性表达的基因有利于产量形成和杂种优势的发生。
