Oh Kyunghee, Cheon Ban Yoon, Cho Sung Hyun, Truong Hoang Quoc, Ok Sung Han, Jeung Ji Ung, Choi Jang Won, Shin Jeong Sheop
Graduate School of Biotechnology, Korea University, Seoul 136-701, Korea.
Transgenic Res. 2003 Jun;12(3):363-7. doi: 10.1023/a:1023321220957.
The bovine growth hormone (bGH) is a natural peptide hormone that controls the differentiation, growth and metabolism, and is produced in the pituitary gland of cows. For the production of bGH from plants, two different bgh clones, of which the pGAbGH1 contaions only mature peptide sequences and the pGAbGH15 contains signal sequences and the first intron, as well as mature peptide sequences, were used. Those bghs under the control of the CaMV 35S promoter and NOS terminator were introduced to tobacco plants via Agrobacterium tumefaciens-mediated transformation. By PCR analyses using bgh and nptII specific primers, 17 and 21 putative transformants were respectively selected from pGAbGH1- and pGAbGH15-transformed tobacco plants. Northern blot analysis showed that the most of the transgenic lines expressed the bgh mRNA. Western blot analysis revealed that the pGAbGH1-transformed tobaccos produced recombinant bGH, but pGAbGH15-transformed ones did not produce the protein. Interestingly, some morphological changes were observed in the roots of transgenic tobacco plants. The transgenic tobacco plants had thick and short roots containing few root hairs in contrast to the non-transformed wild type plants.
牛生长激素（bGH）是一种天然的肽类激素，它控制细胞分化、生长和代谢，由奶牛的垂体产生。为了从植物中生产bGH，使用了两个不同的bGH克隆，其中pGAbGH1仅包含成熟肽序列，pGAbGH15包含信号序列、第一个内含子以及成熟肽序列。那些受花椰菜花叶病毒35S启动子和胭脂碱合成酶终止子控制的bGH通过根癌农杆菌介导的转化被导入烟草植株。通过使用bGH和nptII特异性引物的PCR分析，分别从pGAbGH1转化和pGAbGH15转化的烟草植株中筛选出17个和21个推定的转化体。Northern印迹分析表明，大多数转基因株系表达bGH mRNA。Western印迹分析显示，pGAbGH1转化的烟草产生重组bGH，但pGAbGH15转化的烟草不产生该蛋白。有趣的是，在转基因烟草植株的根部观察到一些形态变化。与未转化的野生型植株相比，转基因烟草植株的根短而粗，根毛较少。
