Mateeva G, Krustev Kh
Eksp Med Morfol. 1976;15(2):99-103.
Fragments the renal cortical part of white rats were frozened in two solutions: saline and 15% of glycerol. The preservation was made in liquid nitrogen (--196 degrees) for a period of 24 hours. The obtained results from the electrone microscopic examination showed that when freezing was performed without protective media, the intactness of the cells and their ultrastructural organization were destroyed. The nuclei preserved their intactness. When protective media (15% of glycerol) was used, then greater stability to low temperatures revealed the epitheleal cells, forming the glomerulus and intersticial tubules, but the most sensitive were the epithelial cells in the main part of the nephrone.
将白色大鼠的肾皮质部分切成碎片，分别置于两种溶液中冷冻：生理盐水和15%的甘油。在液氮（-196摄氏度）中保存24小时。电子显微镜检查得到的结果表明，在没有保护介质的情况下进行冷冻时，细胞的完整性及其超微结构组织被破坏。细胞核保持完整。当使用保护介质（15%的甘油）时，形成肾小球和间质小管的上皮细胞对低温显示出更大的稳定性，但肾单位主要部分的上皮细胞最为敏感。
