Coffey A K, Andrews P M
Transplantation. 1983 Feb;35(2):136-43.
Currently the level of effective (i.e., impermeant) osmotic agent necessary to prevent cell swelling during the cold storage of kidneys is not known. In the present investigation, we used light microscopy and transmission electron microscopy to evaluate the amount of the osmotic agent sucrose which is needed to protect the rat kidney parenchyma from damage over 24 and 48 hr of cold storage. Our observations indicate that when sucrose contributes 140 mOsmol or more to the total osmolality of phosphate-buffered solutions, most cell swelling and associated ultrastructural damage can be prevented over 48 hr of cold storage. We also found little difference between the quality of kidney ultrastructural preservation which results when kidneys are stored in isotonic (300 mOsmol) versus hypertonic (400 mOsmol) solutions that contain the same amount of sucrose. The overall quality of preservation seen with solutions which contain 140 or 200 mOsmol of sucrose is dramatically better than that which we previously observed with such clinically popular kidney preservation solutions as Collins, Euro-Collins, and Sacks.
目前，在肾脏冷藏期间防止细胞肿胀所需的有效（即非渗透性）渗透剂水平尚不清楚。在本研究中，我们使用光学显微镜和透射电子显微镜来评估在24小时和48小时的冷藏过程中，保护大鼠肾实质免受损伤所需的渗透剂蔗糖的量。我们的观察结果表明，当蔗糖对磷酸盐缓冲溶液的总渗透压贡献140毫渗摩尔或更多时，在48小时的冷藏过程中，大多数细胞肿胀和相关的超微结构损伤可以得到预防。我们还发现，将肾脏储存在含有相同量蔗糖的等渗（300毫渗摩尔）与高渗（400毫渗摩尔）溶液中时，肾脏超微结构保存的质量几乎没有差异。含有140或200毫渗摩尔蔗糖的溶液的总体保存质量明显优于我们之前使用柯林斯、欧洲柯林斯和萨克斯等临床上常用的肾脏保存溶液所观察到的质量。
