Nanobashvili Zakaria, Chachua Tamar, Nanobashvili Avtandil, Bilanishvili Irina, Lindvall Olle, Kokaia Zaal
I. Beritashvili Institute of Physiology, Georgian Academy of Sciences, Tbilisi, Republic of Georgia.
Exp Neurol. 2003 Jun;181(2):224-30. doi: 10.1016/s0014-4886(03)00045-1.
Kindling is a model of temporal lobe epilepsy in which repeated electrical stimulations in limbic areas lead to progressive increase of seizure susceptibility, culminating in generalized convulsions and the establishment of a permanent epileptic syndrome. We studied here the effect of stimulations in the thalamic reticular nucleus (TRN) on the development of seizures and hippocampal hyperexcitability in kindling elicited from the ventral hippocampus in rats. Animals given 12 kindling stimulations per day with 30-min intervals for 4 consecutive days developed generalized convulsions on day 4. Stimulations in TRN delivered simultaneously with those in the hippocampus induced marked suppression of seizure generalization. Similarly, the number of generalized seizures and the duration of behavioral convulsions were reduced when rats subjected to 40 kindling stimulations with 5-min intervals during about 3 h were costimulated in the TRN. The anticonvulsant effect of TRN costimulation was detected also when rats were test-stimulated in the hippocampus at 24 h and 2 and 4 weeks after the initial 40 hippocampal stimulations. Our data provide the first evidence that TRN stimulations can act to suppress limbic motor seizures in hippocampal kindling and suggest a new approach for seizure control in temporal lobe epilepsy.
点燃效应是一种颞叶癫痫模型，其中边缘区域的反复电刺激会导致癫痫易感性逐渐增加，最终引发全身性惊厥并形成永久性癫痫综合征。我们在此研究了丘脑网状核（TRN）刺激对大鼠腹侧海马诱发的点燃效应中癫痫发作发展和海马兴奋性过高的影响。每天给予动物12次点燃刺激，间隔30分钟，连续4天，第4天出现全身性惊厥。TRN刺激与海马刺激同时进行可显著抑制癫痫发作的泛化。同样，当大鼠在约3小时内接受40次间隔5分钟的点燃刺激并在TRN中进行共刺激时，全身性癫痫发作的次数和行为性惊厥的持续时间减少。当大鼠在最初40次海马刺激后的24小时以及2周和4周在海马进行测试刺激时，也检测到TRN共刺激的抗惊厥作用。我们的数据首次证明TRN刺激可抑制海马点燃效应中的边缘运动性癫痫发作，并为颞叶癫痫的发作控制提出了一种新方法。
