Zhang Yan, Kimura Tairo, Fujiki Keiko, Sakuma Hitoshi, Murakami Akira, Kanai Atsushi
Department of Ophthalmology, Juntendo University School of Medicine, Tokyo, Japan.
Jpn J Ophthalmol. 2003 May-Jun;47(3):260-4. doi: 10.1016/s0021-5155(03)00014-5.
To detect simultaneously herpes simplex virus type 1 (HSV-1), type 2 (HSV-2), cytomegalovirus (CMV), and varicella-zoster virus (VZV) in ocular specimens suspected of indicating viral infection, and to compare the results of multiplex polymerase chain reaction (PCR) with those of uniplex PCR.
Forty specimens, collected from 33 patients with clinically suspected herpes virus ocular infection, were tested. DNA was extracted from the specimens and amplified by multiplex and uniplex PCR.
Both multiplex PCR and uniplex PCR gave the same results. Nineteen (19/33, 57.6%) patients were PCR-positive, among whom HSV-1 was detected in 13 (13/19, 68.4%) patients, and VZV in 6 (6/19, 31.6%) patients.
These results demonstrated that multiplex PCR is as reliable as uniplex PCR, and is an accurate and a cost-saving method to identify several agents from a single specimen.
同时检测疑似病毒感染的眼部标本中的1型单纯疱疹病毒（HSV-1）、2型单纯疱疹病毒（HSV-2）、巨细胞病毒（CMV）和水痘-带状疱疹病毒（VZV），并比较多重聚合酶链反应（PCR）与单重PCR的结果。
对33例临床疑似疱疹病毒眼部感染患者采集的40份标本进行检测。从标本中提取DNA，并通过多重PCR和单重PCR进行扩增。
多重PCR和单重PCR结果相同。19例（19/33，57.6%）患者PCR检测呈阳性，其中13例（13/19，68.4%）患者检测到HSV-1，6例（6/19，31.6%）患者检测到VZV。
这些结果表明，多重PCR与单重PCR一样可靠，是一种从单个标本中鉴定多种病原体的准确且节省成本的方法。
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