扩张蛋白基因表达的调控影响转基因水稻植株的生长发育。
Regulation of expansin gene expression affects growth and development in transgenic rice plants.
作者信息
Choi Dongsu, Lee Yi, Cho Hyung-Taeg, Kende Hans
机构信息
Michigan State University--Department of Energy Plant Research Laboratory, Michigan State University, East Lansing, Michigan 48824-1312, USA.
出版信息
Plant Cell. 2003 Jun;15(6):1386-98. doi: 10.1105/tpc.011965.
To investigate the in vivo functions of expansins, we generated transgenic rice plants that express sense and antisense constructs of the expansin gene OsEXP4. In adult plants with constitutive OsEXP4 expression, 12% of overexpressors were taller and 88% were shorter than the average control plants, and most overexpressors developed at least two additional leaves. Antisense plants were shorter and flowered earlier than the average control plants. In transgenic plants with inducible OsEXP4 expression, we observed a close correlation between OsEXP4 protein levels and seedling growth. Coleoptile and mesocotyl length increased by up to 31 and 97%, respectively, in overexpressors, whereas in antisense seedlings, they decreased by up to 28 and 43%, respectively. The change in seedling growth resulted from corresponding changes in cell size, which in turn appeared to be a function of altered cell wall extensibility. Our results support the hypothesis that expansins are involved in enhancing growth by mediating cell wall loosening.
为了研究扩展蛋白的体内功能，我们构建了表达扩展蛋白基因OsEXP4正义和反义构建体的转基因水稻植株。在组成型表达OsEXP4的成年植株中，12%的过表达植株比对照植株平均更高，88%的过表达植株比对照植株平均更矮，并且大多数过表达植株至少多发育了两片叶子。反义植株比对照植株平均更矮且开花更早。在诱导型表达OsEXP4的转基因植株中，我们观察到OsEXP4蛋白水平与幼苗生长之间存在密切相关性。在过表达植株中，胚芽鞘和中胚轴长度分别增加了31%和97%，而在反义幼苗中，它们分别减少了28%和43%。幼苗生长的变化是由细胞大小的相应变化引起的，而细胞大小的变化似乎又是细胞壁伸展性改变的结果。我们的结果支持了扩展蛋白通过介导细胞壁松弛来促进生长这一假说。
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