Yu Jun, Hu Songnian, Wang Jun, Wong Gane Ka-Shu, Li Songgang, Liu Bin, Deng Yajun, Dai Li, Zhou Yan, Zhang Xiuqing, Cao Mengliang, Liu Jing, Sun Jiandong, Tang Jiabin, Chen Yanjiong, Huang Xiaobing, Lin Wei, Ye Chen, Tong Wei, Cong Lijuan, Geng Jianing, Han Yujun, Li Lin, Li Wei, Hu Guangqiang, Huang Xiangang, Li Wenjie, Li Jian, Liu Zhanwei, Li Long, Liu Jianping, Qi Qiuhui, Liu Jinsong, Li Li, Li Tao, Wang Xuegang, Lu Hong, Wu Tingting, Zhu Miao, Ni Peixiang, Han Hua, Dong Wei, Ren Xiaoyu, Feng Xiaoli, Cui Peng, Li Xianran, Wang Hao, Xu Xin, Zhai Wenxue, Xu Zhao, Zhang Jinsong, He Sijie, Zhang Jianguo, Xu Jichen, Zhang Kunlin, Zheng Xianwu, Dong Jianhai, Zeng Wanyong, Tao Lin, Ye Jia, Tan Jun, Ren Xide, Chen Xuewei, He Jun, Liu Daofeng, Tian Wei, Tian Chaoguang, Xia Hongai, Bao Qiyu, Li Gang, Gao Hui, Cao Ting, Wang Juan, Zhao Wenming, Li Ping, Chen Wei, Wang Xudong, Zhang Yong, Hu Jianfei, Wang Jing, Liu Song, Yang Jian, Zhang Guangyu, Xiong Yuqing, Li Zhijie, Mao Long, Zhou Chengshu, Zhu Zhen, Chen Runsheng, Hao Bailin, Zheng Weimou, Chen Shouyi, Guo Wei, Li Guojie, Liu Siqi, Tao Ming, Wang Jian, Zhu Lihuang, Yuan Longping, Yang Huanming
Beijing Genomics Institute/Center of Genomics and Bioinformatics, Chinese Academy of Sciences, Beijing 101300, China.
Science. 2002 Apr 5;296(5565):79-92. doi: 10.1126/science.1068037.
We have produced a draft sequence of the rice genome for the most widely cultivated subspecies in China, Oryza sativa L. ssp. indica, by whole-genome shotgun sequencing. The genome was 466 megabases in size, with an estimated 46,022 to 55,615 genes. Functional coverage in the assembled sequences was 92.0%. About 42.2% of the genome was in exact 20-nucleotide oligomer repeats, and most of the transposons were in the intergenic regions between genes. Although 80.6% of predicted Arabidopsis thaliana genes had a homolog in rice, only 49.4% of predicted rice genes had a homolog in A. thaliana. The large proportion of rice genes with no recognizable homologs is due to a gradient in the GC content of rice coding sequences.
我们通过全基因组鸟枪法测序，获得了中国种植最广泛的亚种——水稻（Oryza sativa L. ssp. indica）基因组的草图序列。该基因组大小为466兆碱基，估计有46,022至55,615个基因。组装序列的功能覆盖率为92.0%。约42.2%的基因组由精确的20核苷酸寡聚体重复序列组成，并且大多数转座子位于基因间区域。虽然预测的拟南芥基因中有80.6%在水稻中有同源物，但预测的水稻基因中只有49.4%在拟南芥中有同源物。水稻中大量没有可识别同源物的基因是由于水稻编码序列的GC含量存在梯度。
