Fragoso G, Lamoyi E, Mellor A, Lomelí C, Hernández M, Sciutto E
Department of Immunology, Instituto de Investigaciones Biomédicas, UNAM, México D.F., México.
Infect Immun. 1998 Feb;66(2):760-4. doi: 10.1128/IAI.66.2.760-764.1998.
We previously reported important differences in resistance to Taenia crassiceps murine cysticercosis between BALB/c substrains. It was suggested that resistance might correlate with expression of the nonclassic class I major histocompatibility complex (MHC) Qa-2 antigen; BALB/cAnN is Qa-2 negative and highly susceptible to T. crassiceps, whereas BALB/cJ expresses Qa-2 and is highly resistant. In this study, we investigated the role of Qa-2 in mediating resistance to cysticercosis by linkage analysis and infection of Qa-2 transgenic mice. In BALB/cAnN x (C57BL/6J x BALB/cAnN)F1 and BALB/cAnN x (BALB/cJ x BALB/cAnN)F1 backcrosses, the expression of Qa-2 antigen correlated with resistance to cysticercosis. Significantly fewer parasites were recovered from infected Qa-2 transgenic male and female mice than from nontransgenic mice of similar genetic background. These results clearly demonstrate that the Qa-2 MHC antigen is involved in resistance to T. crassiceps cysticercosis.
我们先前报道了BALB/c亚系小鼠对肥胖带绦虫鼠囊尾蚴病的抗性存在重要差异。有研究表明，抗性可能与非经典I类主要组织相容性复合体（MHC）Qa - 2抗原的表达相关；BALB/cAnN小鼠Qa - 2呈阴性，对肥胖带绦虫高度易感，而BALB/cJ小鼠表达Qa - 2且具有高度抗性。在本研究中，我们通过连锁分析和感染Qa - 2转基因小鼠，研究了Qa - 2在介导对囊尾蚴病抗性中的作用。在BALB/cAnN×（C57BL/6J×BALB/cAnN）F1和BALB/cAnN×（BALB/cJ×BALB/cAnN）F1回交后代中，Qa - 2抗原的表达与对囊尾蚴病的抗性相关。从感染的Qa - 2转基因雄鼠和雌鼠体内回收的寄生虫明显少于具有相似遗传背景的非转基因小鼠。这些结果清楚地表明，Qa - 2 MHC抗原参与了对肥胖带绦虫囊尾蚴病的抗性。
