利用乙醛和磷酸丙糖微生物生产5-磷酸脱氧核糖用于合成2'-脱氧核糖核苷。
Microbial production of 2-deoxyribose 5-phosphate from acetaldehyde and triosephosphate for the synthesis of 2'-deoxyribonucleosides.
作者信息
Ogawa Jun, Saito Kyota, Sakai Takafumi, Horinouchi Nobuyuki, Kawano Takako, Matsumoto Seiichiro, Sasaki Mie, Mikami Yoichi, Shimizu Sakayu
机构信息
Division of Applied Life Sciences, Graduate School of Agriculture, Kyoto University, Kitashirakawa-oiwakecho, Sakyo-ku, Kyoto 606-8502, Japan.
出版信息
Biosci Biotechnol Biochem. 2003 Apr;67(4):933-6. doi: 10.1271/bbb.67.933.
2-Deoxyribose 5-phosphate was produced from acetaldehyde and dihydroxyacetone phosphate via D-glyceraldehyde 3-phosphate by Klebsiella pneumoniae B-4-4 through deoxyriboaldolase- and triosephosphate isomerase-catalyzing reactions. Under the optimum conditions, 98.7 mM 2-deoxyribose 5-phosphate was produced from 200 mM acetaldehyde and 117 mM dihydroxyacetone phosphate in 2 h with a molar yield of 84%. The 2-deoxyriobse 5-phosphate produced was directly transformed to 2'-deoxyribonucleoside by phosphopentomutase- and nucleoside phosphorylase-catalyzing reactions.
肺炎克雷伯菌B-4-4通过脱氧核糖醛缩酶和磷酸丙糖异构酶催化的反应，由乙醛和磷酸二羟丙酮经3-磷酸-D-甘油醛生成5-磷酸-2-脱氧核糖。在最佳条件下，200 mM乙醛和117 mM磷酸二羟丙酮在2小时内生成了98.7 mM的5-磷酸-2-脱氧核糖，摩尔产率为84%。生成的5-磷酸-2-脱氧核糖通过磷酸戊糖变位酶和核苷磷酸化酶催化的反应直接转化为2'-脱氧核糖核苷。
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