Biokatalyse, Afdeling Biotechnologie, Technische Universiteit Delft, Van der Maasweg 9, 2629 HZ, Delft, The Netherlands.
Chemistry Department, Faculty of Science, Sohag University, Sohag, 82524, Egypt.
Appl Microbiol Biotechnol. 2018 Dec;102(23):9959-9971. doi: 10.1007/s00253-018-9392-8. Epub 2018 Oct 3.
2-Deoxy-D-ribose-5-phosphate aldolase (DERA) is a class I aldolase that offers access to several building blocks for organic synthesis. It catalyzes the stereoselective C-C bond formation between acetaldehyde and numerous other aldehydes. However, the practical application of DERA as a biocatalyst is limited by its poor tolerance towards industrially relevant concentrations of aldehydes, in particular acetaldehyde. Therefore, the development of proper experimental conditions, including protein engineering and/or immobilization on appropriate supports, is required. The present review is aimed to provide a brief overview of DERA, its history, and progress made in understanding the functioning of the enzyme. Furthermore, the current understanding regarding aldehyde resistance of DERA and the various optimizations carried out to modify this property are discussed.
2-脱氧-D-核糖-5-磷酸醛缩酶（DERA）是一种 I 类醛缩酶，为有机合成提供了多种构建块。它催化乙醛和许多其他醛之间的立体选择性 C-C 键形成。然而，DERA 作为生物催化剂的实际应用受到其对工业相关浓度醛（特别是乙醛）的耐受性差的限制。因此，需要开发适当的实验条件，包括蛋白质工程和/或在适当的载体上固定化。本综述旨在简要概述 DERA，包括其历史和在理解酶功能方面取得的进展。此外，还讨论了目前对 DERA 醛抗性的理解以及为改变这种特性而进行的各种优化。
