Stux S V, Ovary Z
Int Arch Allergy Appl Immunol. 1976;51(3):338-58. doi: 10.1159/000231608.
Homologous skin sensitizing antibodies in the rabbit can be differentiated by their requirement for complement in expressing the passive cutaneous anaphylactic reaction as complement dependent (CD) or complement independent (CI). CI antibodies are representative of the IgE class, while the CD antibodies belong to a proposed new subclass of IgG designated IgGa (a = anaphylaxis). The following are characteristics of CI (IgE) antibodies: elution at 0.05 M on DEAE at pH 8.1 migration ahead of 7S in ultracentrifugation, molecular weight 215,000 daltons by Sephadex G-200, fast gamma-electrophoretic mobility, isoelectric point, pI = 4.98 (5.68-4.58), 85-95% heat sensitivity, sensitivity to reduction plus alkylation and option at 0.01 M on DEAE, 7S in ultracentrifugation, MW 138,000 daltons, slow gamma-electrophoretic mobility pI = 6.90 (8.30-5.70), resistance to heating and to reduction plus alkylation and 1-day optimal SP. CI antibodies could be absorbed by anti-Fab, anti-epsilon, the homologous antigen and were not absorbed by anti-gamma, anti-alpha, or heterologous antigens. The CD antibodies were absorbed by anti-Fab, anti-gamma and not absorbed by anti-epsilon, anti-alpha, or heterologous antigens.
兔体内的同源皮肤致敏抗体可根据其在表达被动皮肤过敏反应时对补体的需求分为补体依赖型（CD）或补体非依赖型（CI）。CI抗体是IgE类的代表，而CD抗体属于提议的IgG新亚类，称为IgGa（a =过敏反应）。以下是CI（IgE）抗体的特征：在pH 8.1的DEAE上以0.05 M洗脱，在超速离心中迁移速度快于7S，通过Sephadex G - 200测得分子量为215,000道尔顿，γ电泳迁移速度快，等电点pI = 4.98（5.68 - 4.58），85 - 95%对热敏感，对还原加烷基化敏感，在DEAE上以0.01 M洗脱，超速离心中为7S，分子量138,000道尔顿，γ电泳迁移速度慢，pI = 6.90（8.30 - 5.70），对加热和还原加烷基化有抗性，最佳致敏期为1天。CI抗体可被抗Fab、抗ε、同源抗原吸收，而不被抗γ、抗α或异源抗原吸收。CD抗体可被抗Fab、抗γ吸收，而不被抗ε、抗α或异源抗原吸收。
