Ovary Z, Kaplan B, Kojima S
Int Arch Allergy Appl Immunol. 1976;51(4):416-28. doi: 10.1159/000231616.
Anti-dinitrophenyl (DNP) IgE antibody was produced in guinea pigs (GP) by immunization and repeated boosters with 1 mug DNP-Ascaris extract in A1 (OH)3. The isoelectric point was determined to be 6.48 and the molecular weight to be 185,000 daltons. In absorbtion chromatography, rabbit anti-GPIgG1 could not absorb the anaphylactic activity of the IgE. The activity of IgE was destroyed by heating at 56 degrees C for 1 h. IgE persisted in the skin for at least 3 weeks.
通过用1微克二硝基苯 - 蛔虫提取物与氢氧化铝（A1(OH)3）免疫并重复加强免疫，在豚鼠（GP）中产生了抗二硝基苯（DNP）IgE抗体。其等电点测定为6.48，分子量为185,000道尔顿。在吸附色谱中，兔抗豚鼠IgG1不能吸附IgE的过敏活性。IgE的活性在56℃加热1小时后被破坏。IgE在皮肤中持续至少3周。
