Vasků A, Soucek M, Goldbergová M, Vácha J
Institute of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Physiol Res. 2003;52(3):291-7.
The aim of the study was to assess the association between promoter polymorphism [A(-596)G] in interleukin-6 gene and office systolic and diastolic blood pressures, and the heart rate (HR) in apparently healthy Czech subjects. Furthermore, we evaluated the possible influence of gender, BMI and smoking on these supposed associations. An age-matched (40-50 years) and gender-matched (F/M=81/89) sample of apparently healthy Czech subjects (n=170, F/M=81/89) without hypertension, other cardiovascular diseases or diabetes was examined. The A(-596)G Il-6 gene polymorphism was detected by the PCR method. No differences in genotype distribution and/or allelic frequency was found between groups with lower systolic blood pressure (Ł 122 mm Hg) and higher systolic blood pressure (> 122 mm Hg). Similarly, no differences in the IL-6 polymorphism were found between lower (Ł 86 mm Hg) and higher (> 86 mm Hg) diastolic blood pressure groups. However, we proved a significant increase of genotypes AG+GG as well as the allele (-596)G in higher (>78 beats/min) heart rate group. The genotypes AG+GG represent significantly higher relative risk for higher HR frequency, especially in women. Among lean persons with a low heart rate frequency, fewer AG+GG genotypes were determined than among any other subjects. The genotypes AG+GG are more frequent in non-smoking persons with higher HR compared to non-smoking subjects with lower HR, especially in women. Gender, BMI and smoking substantially modify the distribution of A(-596)G Il-6 gene polymorphism in apparently healthy persons with lower or higher heart rate.
本研究旨在评估白细胞介素-6基因启动子多态性[A(-596)G]与表面健康的捷克受试者的诊室收缩压和舒张压以及心率(HR)之间的关联。此外，我们评估了性别、体重指数和吸烟对这些假定关联的可能影响。对年龄匹配(40-50岁)和性别匹配(F/M = 81/89)的表面健康的捷克受试者样本(n = 170, F/M = 81/89)进行了检查，这些受试者无高血压、其他心血管疾病或糖尿病。采用聚合酶链反应(PCR)方法检测A(-596)G Il-6基因多态性。收缩压较低(≤122 mmHg)和收缩压较高(>122 mmHg)的组之间，未发现基因型分布和/或等位基因频率存在差异。同样，舒张压较低(≤86 mmHg)和舒张压较高(>86 mmHg)的组之间，未发现IL-6多态性存在差异。然而，我们证实，心率较高(>78次/分钟)组的AG+GG基因型以及(-596)G等位基因显著增加。AG+GG基因型代表较高心率频率的相对风险显著更高，尤其是在女性中。在心率频率较低的瘦人中，AG+GG基因型的检出率低于其他任何受试者。与心率较低的非吸烟受试者相比，心率较高的非吸烟受试者中AG+GG基因型更常见，尤其是在女性中。性别、体重指数和吸烟显著改变了心率较低或较高的表面健康人群中A(-596)G Il-6基因多态性的分布。
