Yoda Tomoko, Suzuki Yasuhiko, Terano Yoshitake, Yamazaki Kenji, Sakon Naomi, Kuzuguchi Tsuyoshi, Oda Hajime, Tsukamoto Teizo
Division of Food Microbiology, Osaka Prefectural Institute of Public Health, Japan.
J Clin Microbiol. 2003 Jun;41(6):2367-71. doi: 10.1128/JCM.41.6.2367-2371.2003.
We have been characterizing monoclonal antibodies against Norovirus (Norwalk-like virus). In the course of our study, two monoclonal antibodies generated against Norovirus genogroup II capsid protein were found to react not only to genogroup II but also to genogroup I recombinant capsid proteins. In addition, we showed that these two monoclonal antibodies reacted to a 40-amino-acid-fragment located close to the N-terminal region of genogroup II Norovirus. Similar reactivity was observed with the equivalent region of genogroup I Norovirus. In this study, we confirmed that the epitopes of the two monoclonal antibodies existed within an 11-amino-acid peptide. To obtain an idea of the reactive ranges of the two monoclonal antibodies toward different strains of Norovirus, their reactivities were investigated using 16 types of peptide constructed according to the data in GenBank and 8 recombinant capsid proteins (7 whole capsid proteins and 1 short [80-amino-acid] protein fragment). A characteristic broad reactivity of the two monoclonal antibodies is clearly shown by the results of this study. Thus, these monoclonal antibodies could be useful tools for detecting a broad range of Norovirus strains.
我们一直在对针对诺如病毒（诺沃克样病毒）的单克隆抗体进行特性分析。在我们的研究过程中，发现两种针对诺如病毒基因组II衣壳蛋白产生的单克隆抗体不仅能与基因组II反应，还能与基因组I重组衣壳蛋白反应。此外，我们还表明这两种单克隆抗体能与位于基因组II诺如病毒N端区域附近的一个40个氨基酸的片段发生反应。在基因组I诺如病毒的等效区域也观察到了类似的反应性。在本研究中，我们证实这两种单克隆抗体的表位存在于一个11个氨基酸的肽段内。为了了解这两种单克隆抗体对不同诺如病毒株的反应范围，我们根据GenBank中的数据构建了16种肽段，并使用8种重组衣壳蛋白（7种全衣壳蛋白和1种短的[80个氨基酸]蛋白片段）研究了它们的反应性。这项研究的结果清楚地显示了这两种单克隆抗体具有特征性的广泛反应性。因此，这些单克隆抗体可能是检测多种诺如病毒株的有用工具。
