在胃肠炎暴发病例标本中检测重组诺沃克病毒抗原抗体
Detection of antibody to recombinant Norwalk virus antigen in specimens from outbreaks of gastroenteritis.
作者信息
Monroe S S, Stine S E, Jiang X, Estes M K, Glass R I
机构信息
Viral Gastroenteritis Section, Centers for Disease Control and Prevention, Atlanta, Georgia 30333.
出版信息
J Clin Microbiol. 1993 Nov;31(11):2866-72. doi: 10.1128/jcm.31.11.2866-2872.1993.
Abstract
Norwalk virus and other small round-structured viruses are commonly associated with outbreaks of gastroenteritis. We used a recently described recombinant-expressed Norwalk virus (rNV) capsid protein in enzyme immunoassays to quantitatively measure immunoglobulin G (IgG) and IgA to Norwalk virus in serum pairs from patients involved in outbreaks of gastroenteritis. The outbreaks previously were classified, on the basis of the results of a blocking antibody assay, as Norwalk virus negative, serologically intermediate, or Norwalk virus positive. The rNV IgG assay was more sensitive than the blocking assay for detecting IgG to Norwalk virus in serum from patients in all outbreak classes. There was 79% concordance between seroconversions detected by the blocking antibody assay and those detected by the rNV IgG assay. The rNV IgA assay detected seroconversions to Norwalk virus primarily in patients involved in outbreaks previously classified as Norwalk virus positive.
摘要
诺如病毒和其他小圆形结构病毒通常与肠胃炎暴发有关。我们在酶免疫测定中使用了最近描述的重组表达诺如病毒（rNV）衣壳蛋白，以定量测量参与肠胃炎暴发的患者血清对中针对诺如病毒的免疫球蛋白G（IgG）和IgA。此前，这些暴发根据阻断抗体检测结果被分类为诺如病毒阴性、血清学中间型或诺如病毒阳性。rNV IgG检测在检测所有暴发类别患者血清中针对诺如病毒的IgG方面比阻断检测更敏感。阻断抗体检测和rNV IgG检测所检测到的血清转化之间存在79%的一致性。rNV IgA检测主要在先前分类为诺如病毒阳性的暴发所涉及的患者中检测到针对诺如病毒的血清转化。
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