Heikkilä Tero, Huppertz Hans-Iko, Seppälä Ilkka, Sillanpää Heidi, Saxen Harri, Lahdenne Pekka
Hospital for Children and Adolescents, University of Helsinki, Helsinki, Finland.
J Infect Dis. 2003 Jun 15;187(12):1888-94. doi: 10.1086/375371. Epub 2003 Jun 4.
The performance of ELISAs with the recombinant antigens decorin-binding protein A (DbpA), DbpB, and BBK32 (from Borrelia afzelii, B. garinii, and B. burgdorferi sensu stricto) and VlsE peptide antigen invariable region 6 (IR(6)) were evaluated in the serodiagnosis and follow-up of children with Lyme arthritis (LA). Serum samples were obtained from 52 children with clinically typical and serologically confirmed LA. In IgG ELISAs, at diagnosis, 50 samples were positive for BBK32, 51 for DbpA, 40 for DbpB, and 51 for IR(6). In the posttreatment follow-up, the rate of decline of the antibodies to the recombinant protein antigens or to IR(6) did not appear useful in the prediction of the treatment response or the clinical course of LA. Yet, IR(6) seems to have the greatest potential to be used universally in the diagnostic serology of Lyme borreliosis (LB). Alternate to that, the use of several specific borrelial antigens, in parallel, might improve the accuracy of serology for LB.
对采用重组抗原核心蛋白聚糖结合蛋白A（DbpA）、DbpB和BBK32（分别来自阿氏疏螺旋体、伽氏疏螺旋体和狭义伯氏疏螺旋体）以及VlsE肽抗原可变区6（IR(6)）的酶联免疫吸附测定（ELISA）在莱姆关节炎（LA）患儿血清学诊断及随访中的表现进行了评估。从52例临床症状典型且血清学确诊为LA的患儿中获取血清样本。在IgG ELISA检测中，诊断时，50份样本对BBK32呈阳性，51份对DbpA呈阳性，40份对DbpB呈阳性，51份对IR(6)呈阳性。在治疗后随访中，针对重组蛋白抗原或IR(6)的抗体下降速率在预测LA的治疗反应或临床病程方面似乎并无作用。然而，IR(6)似乎在莱姆病（LB）诊断血清学中具有最广泛的应用潜力。除此之外，同时使用多种特异性疏螺旋体抗原可能会提高LB血清学检测的准确性。
