Lahdenne Pekka, Sarvas Heikki, Kajanus Riikka, Eholuoto Miia, Sillanpää Heidi, Seppälä Ilkka
Hospital for Children and Adolescents, University of Helsinki, Stenbäckinkatu 11, FIN-00290 Helsinki, Finland.
HUSLAB Laboratory Diagnostics, Helsinki University Central Hospital, Haartmaninkatu 3, FIN-00290 Helsinki, Finland.
J Med Microbiol. 2006 Nov;55(Pt 11):1499-1504. doi: 10.1099/jmm.0.46621-0.
Recombinantly produced borrelial BBK32 protein fragments originating from Borrelia burgdorferi sensu stricto, Borrelia garinii and Borrelia afzelii were evaluated as antigens in the serology of Lyme borreliosis (LB). In ELISA, a mid-portion hydrophilic fragment reacted with LB patient sera. Of the 23 patients with culture- or PCR-positive erythema migrans (EM), 43 % at diagnosis and 52 % at convalescence were positive for at least one Borrelia species-specific variant BBK32 fragment antigen. In parallel ELISAs with BBK32 whole proteins from the three borrelial subspecies as antigens, 17 % at diagnosis and 26 % at convalescence were positive. These results suggest that BBK32 protein fragments may improve the early IgG serology of LB compared to the BBK32 whole protein.
对重组产生的源自狭义伯氏疏螺旋体、伽氏疏螺旋体和阿氏疏螺旋体的疏螺旋体BBK32蛋白片段作为莱姆病（LB）血清学中的抗原进行了评估。在酶联免疫吸附测定（ELISA）中，一个中部亲水性片段与LB患者血清发生反应。在23例培养或聚合酶链反应（PCR）呈阳性的游走性红斑（EM）患者中，43%在诊断时以及52%在恢复期至少对一种疏螺旋体物种特异性变体BBK32片段抗原呈阳性。在以三个疏螺旋体亚种的BBK32全蛋白作为抗原的平行ELISA中，诊断时17%呈阳性，恢复期26%呈阳性。这些结果表明，与BBK32全蛋白相比，BBK32蛋白片段可能会改善LB的早期IgG血清学检测。
