Zajkowska Joanna, Kondrusik Maciej, Pancewicz Sławomir, Grygorczuk Sambor, Swierzbińska Renata, Hermanowska-Szpakowicz Teresa, Czeczuga Anna, Sienkiewicz Iwona
Klinika Chorób Zakainych i Neuroinfekcji AM w Białymstoku.
Przegl Epidemiol. 2006;60 Suppl 1:177-85.
The aim of the study was the evaluation of the efficiency of Western blot (EcoLine) test detecting simoultanous presence of IgM and IgG antibodies against B. burgdorferi in diagnosis of early and late stage of Lyme borreliosis. The comparison of results achieved by performing test Western-blot, ELISA (based on recombinant antigens of three genospecies of Borrelia) and EIA (based on antigens of one B. burgdorferi genospecies). The tests Western blot: EcoLine (Virotech) with antygens "in vivo", ELISA Borrelia IgM, IgG recombinant (Biomedica), EIA: B. b. ss. IgG, EIA B. garinii IgG, EIA B. afzelii IgG (TestLine) were used. Results showed efficacy of detecting IgM, IgG antibodies against VlsE simultanously and IgG antibodies against "in vivo" antigens in diagnosis of early stages of Lyme disease when atypical picture skin lessions arise diagnostic doubts and in discerning early and late stage of disease. The EIA tests based on one B. burgdoreferi genospecies seem less effective in comparison to ELISA tests based on 3 genospecies antigens.
本研究的目的是评估蛋白质印迹法（EcoLine）检测抗伯氏疏螺旋体IgM和IgG抗体同时存在对莱姆病早期和晚期诊断的效率。比较蛋白质印迹法、酶联免疫吸附测定（基于伯氏疏螺旋体三个基因种的重组抗原）和酶免疫测定（基于一种伯氏疏螺旋体基因种的抗原）所获得的结果。使用了以下检测方法：带有“体内”抗原的蛋白质印迹法：EcoLine（Virotech）、伯氏疏螺旋体IgM、IgG重组酶联免疫吸附测定（Biomedica）、酶免疫测定：伯氏疏螺旋体狭义亚种IgG、伽氏疏螺旋体IgG、阿氏疏螺旋体IgG（TestLine）。结果表明，在莱姆病早期出现非典型皮肤病变诊断存疑时，以及在区分疾病的早期和晚期时，检测抗VlsE的IgM、IgG抗体以及抗“体内”抗原的IgG抗体具有诊断效力。与基于三种基因种抗原的酶联免疫吸附测定相比，基于一种伯氏疏螺旋体基因种的酶免疫测定似乎效果较差。
