Chen Bing-Qing, Yang Yan-Mei, Gao Yan-Hui, Liu Jia-Ren, Xue Ying-Ben, Wang Xuan-Lin, Zheng Yu-Mei, Zhang Jing-Shu, Liu Rui-Hai
Department of Nutrition and Food Hygiene, Public Health College, Harbin Medical University, 199 Dongdazhi Street, Nangang District, Harbin 150001, Heilongjiang Province, China.
World J Gastroenterol. 2003 Sep;9(9):1909-14. doi: 10.3748/wjg.v9.i9.1909.
To investigate the effect of c9, t11-conjugated linoleic acid (c9, t11-CLA) on the invasion of human gastric carcinoma cell line and its possible mechanism of preventing metastasis.
Using reconstituted basement membrane invasion, chemotaxis, adhesion, PAGE substrate zymography and RT-PCR assays, we analyzed the abilities of invasion, direct migration, adhesion of intracellular matrix, as well as the activity of type IV collagenase and expression of tissue inhibitor of metalloproteinase (TIMP)-1 and TIMP-2 mRNA in SGC-7901 cells which were treated with gradually increased concentrations (25, 50, 100 and 200 micromol/L) of c9, t11-CLA for 24 h.
At the concentrations of 200 micromol/L, 100 micromol/L and 50 micromol/L, c9,t11-CLA suppressed the invasion of SGC-7901 cells into the reconstituted basement membrane by 53.7 %, 40.9 % and 29.3 %, respectively, in comparison with the negative control. Only in the 200 micromol/L c9,t11-CLA group, the chemotaxis of SGC-7901 cells was inhibited by 16.0 % in comparison with the negative control. C9,t11-CLA also could inhibit the adhesion of SGC-7901 cells to laminin, fibronectin and Matrigel, increase the expression of TIMP-1 and TIMP-2 mRNA, and reduce type IV collagenase activities in the serum-free medium supernatant of SGC-7901 cells.
c9,t11-CLA can inhibit the invasion of SGC-7901 cells at multiple procedures in tumor metastasis cascade, which may be associated with the induction of TIMP-1 and TIMP-2 mRNA expression.
研究c9，t11-共轭亚油酸（c9，t11-CLA）对人胃癌细胞系侵袭的影响及其预防转移的可能机制。
采用重组基底膜侵袭、趋化性、黏附、PAGE底物酶谱分析及逆转录-聚合酶链反应（RT-PCR）检测，分析用逐渐增加浓度（25、50、100和200μmol/L）的c9，t11-CLA处理24小时后的SGC-7901细胞的侵袭能力、直接迁移能力、细胞内基质黏附能力，以及IV型胶原酶活性和金属蛋白酶组织抑制剂（TIMP）-1和TIMP-2 mRNA的表达。
与阴性对照相比，在200μmol/L、100μmol/L和50μmol/L浓度下，c9，t11-CLA分别抑制SGC-7901细胞向重组基底膜的侵袭达53.7%、40.9%和29.3%。仅在200μmol/L c9，t11-CLA组中，SGC-7901细胞的趋化性与阴性对照相比受到16.0%的抑制。c9，t11-CLA还可抑制SGC-7901细胞与层粘连蛋白、纤连蛋白和基质胶的黏附，增加TIMP-1和TIMP-2 mRNA的表达，并降低SGC-7901细胞无血清培养基上清液中的IV型胶原酶活性。
c9，t11-CLA可在肿瘤转移级联反应的多个步骤中抑制SGC-7901细胞的侵袭，这可能与诱导TIMP-1和TIMP-2 mRNA表达有关。
