从龙虾嗅觉器官中克隆G蛋白αi亚基的分子过程
Molecular cloning of a G-protein alpha i subunit from the lobster olfactory organ.
作者信息
McClintock T S, Byrnes A P, Lerner M R
机构信息
Department of Internal Medicine, Yale University School of Medicine, New Haven, CT 06510.
出版信息
Brain Res Mol Brain Res. 1992 Jul;14(3):273-6. doi: 10.1016/0169-328x(92)90183-c.
PMID:1279345
Abstract
A G-protein alpha subunit was cloned from a lobster olfactory organ cDNA library and sequenced. The clone encodes an alpha i subunit based on the 80% identity its predicted amino acid sequence shares with mammalian alpha i subunits. On Northern blots of polyadenylated RNA, the clone hybridized to a 5 kb species from several tissues.
摘要
从龙虾嗅觉器官cDNA文库中克隆并测序了一种G蛋白α亚基。根据其预测的氨基酸序列与哺乳动物αi亚基80%的同一性，该克隆编码一个αi亚基。在聚腺苷酸化RNA的Northern印迹上，该克隆与来自多个组织的5 kb条带杂交。
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