供体血液中血细胞的 BK 和 JC 多瘤病毒 DNA 感染。
Polyomaviruses BK and JC DNA infection in peripheral blood cells from blood donors.
机构信息
Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran; Shiraz Islamic Azad University, Department of Microbiology, Shiraz, Iran.
Shiraz University of Medical Sciences, School of Paramedical Sciences, Diagnostic Laboratory Sciences and Technology Research Center, Shiraz, Iran.
出版信息
Braz J Infect Dis. 2019 Jan-Feb;23(1):22-26. doi: 10.1016/j.bjid.2019.01.005. Epub 2019 Feb 23.
OBJECTIVES
To investigate the prevalence of human polyomavirus (BK and JC viruses) infection in peripheral blood mononuclear cells of healthy blood donors.
METHODS
The study included 250 healthy blood donors. Five-milliliter blood was drawn into sterile EDTA tubes and PBMCs were isolated from whole blood. The isolated PBMCs were counted and stored at -70°C for future investigation. DNA was extracted and subjected to simple, sensitive and specific semi-nested PCR as well as QPCR using both general and specific primers for different assays.
RESULTS
Of 250 blood samples, 66 (26.4%) were positive for BKV DNA (146-34,514 copies/10 cells). JC DNA was found in 45 (18%) blood samples (65-21,250 copies/10 cells). Co-infection with these viruses were found in 11 (4.4%) out of 250 blood samples.
DISCUSSION
Our study provides important data on polyomavirus infection in peripheral blood mononuclear leukocytes in immunocompetent individuals. These data indicate significant differences between the prevalence of BKV and JCV infection in healthy blood donors. The prevalence of BK and JC virus infection is higher in the age range 30-39 years compared to other age ranges.
目的
研究健康献血者外周血单个核细胞中人多瘤病毒（BK 和 JC 病毒）感染的流行情况。
方法
本研究纳入了 250 名健康献血者。无菌 EDTA 管抽取 5 毫升血液，从全血中分离外周血单个核细胞。分离的 PBMC 计数后储存于-70°C，以备将来研究。提取 DNA，采用通用和特异性引物进行简单、敏感和特异性的半巢式 PCR 以及 QPCR 检测。
结果
在 250 份血样中，66 份（26.4%）BKVDNA 阳性（146-34,514 拷贝/10 个细胞）。45 份（18%）血样中发现 JC DNA（65-21,250 拷贝/10 个细胞）。250 份血样中有 11 份（4.4%）存在这两种病毒的合并感染。
讨论
我们的研究提供了免疫功能正常个体外周血单个核白细胞中多瘤病毒感染的重要数据。这些数据表明，健康献血者中 BK 和 JCV 感染的流行率存在显著差异。与其他年龄组相比，30-39 岁年龄组 BK 和 JC 病毒感染的流行率更高。
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