Tseng C E, Yeh C M, Fang C Y, Shay J, Chen P L, Lin M C, Chang D, Wang M
Department of Anatomic Pathology, Buddhist Dalin Tzu Chi General Hospital, Chiayi, Taiwan.
Eur J Clin Microbiol Infect Dis. 2014 Apr;33(4):665-72. doi: 10.1007/s10096-013-2010-x. Epub 2013 Nov 21.
Previous studies have demonstrated that infection with human polyomavirus, such as JCPyV and BKPyV, might be associated with various human tumors. However, an association between human JCPyV and BKPyV infection and diffuse large B-cell lymphoma (DLBCL) has not been reported. The purpose of this study was to examine DLBCLs of the gastrointestinal tract for evidence of human polyomavirus infection. Nested PCR and DNA sequencing were employed for viral DNA detection and viral genotype identification. In addition, two viral proteins, the large tumor antigen (LT) and the major structural protein (VP1), were detected by immunohistochemistry (IHC). Human JCPyV and BKPyV DNA was detected in 14 out of 16 tissue samples (87.5%), whereby nine cases were infected with JCPyV and five cases were infected with BKPyV. Both archetypal and rearranged genotypes of JCPyV and BKPyV were detected in the tissues. LT was detected in 11 tissue samples (68.75%). However, VP1 was not detected in any of the tissue samples. The presence of human JCPyV and BKPyV DNA and protein in DLBCL tissues of gastrointestinal tract were first reported in this study. The current results provide evidence of a possible association between human JCPyV and BKPyV infection and DLBCL.
先前的研究表明，感染人类多瘤病毒，如JC多瘤病毒（JCPyV）和BK多瘤病毒（BKPyV），可能与多种人类肿瘤有关。然而，尚未有关于人类JCPyV和BKPyV感染与弥漫性大B细胞淋巴瘤（DLBCL）之间关联的报道。本研究的目的是检测胃肠道DLBCL中人类多瘤病毒感染的证据。采用巢式PCR和DNA测序进行病毒DNA检测和病毒基因型鉴定。此外，通过免疫组织化学（IHC）检测两种病毒蛋白，即大肿瘤抗原（LT）和主要结构蛋白（VP1）。在16个组织样本中的14个（87.5%）检测到人类JCPyV和BKPyV DNA，其中9例感染JCPyV，5例感染BKPyV。在组织中检测到JCPyV和BKPyV的原型和重排基因型。在11个组织样本（68.75%）中检测到LT。然而，在任何组织样本中均未检测到VP1。本研究首次报道了胃肠道DLBCL组织中存在人类JCPyV和BKPyV DNA及蛋白。目前的结果提供了人类JCPyV和BKPyV感染与DLBCL之间可能存在关联的证据。
