Yeh Feng-Tsgh, Wu Chun-Hsiung, Lee Hong-Zin
Graduate Institute of Chinese Pharmaceutical Science, China Medical College, Taichung, Taiwan.
Int J Cancer. 2003 Aug 10;106(1):26-33. doi: 10.1002/ijc.11185.
Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is an active component from the root and rhizome of Rheum palmatum that has been reported to exhibit antitumor effects through an unknown mechanism. Our study investigated the mechanisms of aloe-emodin-induced cell death in the human lung nonsmall cell carcinoma cell line H460. Aloe-emodin (40 microM)-induced apoptosis of H460 cells involves modulation of cAMP-dependent protein kinase, protein kinase C, Bcl-2, caspase-3 and p38 protein expression. The relationship of various signals involved in cell death, such as cAMP-dependent protein kinase, protein kinase C, Bcl-2, caspase-3 and p38, has been investigated in the regulation of apoptotic cell death of aloe-emodin. We demonstrated that the expression of p38 is an important determinant of apoptotic death induced by aloe-emodin.
芦荟大黄素（1,8 - 二羟基 - 3 -（羟甲基）- 蒽醌）是掌叶大黄根及根茎中的一种活性成分，据报道其可通过未知机制发挥抗肿瘤作用。我们的研究调查了芦荟大黄素诱导人肺非小细胞癌细胞系H460细胞死亡的机制。芦荟大黄素（40微摩尔）诱导H460细胞凋亡涉及对环磷酸腺苷依赖性蛋白激酶、蛋白激酶C、Bcl - 2、半胱天冬酶 - 3和p38蛋白表达的调节。在芦荟大黄素诱导的凋亡细胞死亡调节过程中，已对参与细胞死亡的各种信号（如环磷酸腺苷依赖性蛋白激酶、蛋白激酶C、Bcl - 2、半胱天冬酶 - 3和p38）之间的关系进行了研究。我们证明p38的表达是芦荟大黄素诱导凋亡死亡的一个重要决定因素。
