Lee Hong-Zin, Wu Chun-Hsiung, Chang Shen-Peng
School of Pharmacy, China Medical University, Taichung, Taiwan.
Int J Cancer. 2005 Mar 1;113(6):971-6. doi: 10.1002/ijc.20676.
Aloe-emodin (1,8-dihydroxy-3-(hydroxymethyl)-anthraquinone) is one of the active constituents from the root and rhizome of Rheum palmatum. Our previous study has demonstrated that aloe-emodin induced a significant change in the expression of lung cancer cell apoptosis-related proteins compared to those of control cells. However, the molecular mechanisms underlying the biological effects of aloe-emodin still remain unknown. Based on these reasons, we were interested in the change of aloe-emodin-induced total protein expression by the proteomics technique during aloe-emodin-induced lung cancer cell apoptosis. Our study applied 2D electrophoresis to analyze the proteins involved in aloe-emodin-induced apoptosis in H460 cells. We found that the release of nucleophosmin from the nucleus to the cytosol and the degradation of nucleophosmin were associated with aloe-emodin-induced H460 cell apoptosis. Our study also demonstrated that the gene expression of nucleophosmin remained unchanged after treatment with aloe-emodin. The aloe-emodin-caused increase in the amount of proform and fragment of nucleophosmin in cytoplasm may be one of the important events for aloe-emodin-induced H460 cell apoptosis.
芦荟大黄素（1,8 - 二羟基 - 3 - （羟甲基） - 蒽醌）是掌叶大黄根及根茎中的活性成分之一。我们之前的研究表明，与对照细胞相比，芦荟大黄素可引起肺癌细胞凋亡相关蛋白表达的显著变化。然而，芦荟大黄素生物学效应的分子机制仍不清楚。基于这些原因，我们对蛋白质组学技术在芦荟大黄素诱导肺癌细胞凋亡过程中所引起的总蛋白表达变化感兴趣。我们的研究应用二维电泳分析参与芦荟大黄素诱导H460细胞凋亡的蛋白质。我们发现核磷蛋白从细胞核释放到细胞质以及核磷蛋白的降解与芦荟大黄素诱导的H460细胞凋亡有关。我们的研究还表明，用芦荟大黄素处理后核磷蛋白的基因表达保持不变。芦荟大黄素导致细胞质中核磷蛋白前体和片段数量增加可能是芦荟大黄素诱导H460细胞凋亡的重要事件之一。
