Wasalathanthri Sudharshani, Tennekoon Kamani H, Sufi S
Department of Physiology, Faculty of Medicine, University of Colombo.
Ceylon Med J. 2003 Mar;48(1):4-6. doi: 10.4038/cmj.v48i1.3384.
Ovarian activity should ideally be assessed by serial non-invasive methods that require simple procedures for sample collection and storage. Measurement of urinary oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide is a non-invasive method available for assessment of ovarian activity, but transport of large numbers of urine samples is cumbersome and samples need to be stored frozen. An alternative sample collection, transport and storage procedure that is easier to handle and requires no or minimal cold storage facilities will particularly benefit studies in which ovulatory activity needs to be assessed in field settings.
To evaluate the feasibility of using paper impregnated with urine as an alternative to liquid urine for the measurement of oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide concentrations in the assessment of ovarian activity.
Urine samples collected daily throughout regular menstrual cycles were stored as liquid urine at -20 degrees C, and as paper impregnated with urine, in the refrigerator for 3 to 12 months or at room temperature for 1 to 6 months. Oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide concentrations were measured in these urine samples by enzyme immunoassay. Values obtained were correlated using Spearman's correlation test.
The pattern of oestrone-3 glucuronide and pregnanediol-3 alpha-glucuronide concentrations estimated using paper impregnated with urine followed that of liquid urine in all storage conditions used. Values obtained by two methods correlated significantly (p < 0.001 to 0.0001) though the paper impregnated with urine gave slightly higher values.
Paper impregnated with urine can be used to facilitate sample collection, transport and storage of urine when oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide measurements are required in a large number of serial samples to assess ovarian activity.
理想情况下，卵巢活动应通过一系列非侵入性方法进行评估，这些方法需要简单的样本采集和储存程序。尿雌酮-3-葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷的测定是一种可用于评估卵巢活动的非侵入性方法，但大量尿液样本的运输很麻烦，且样本需要冷冻保存。一种更易于操作且无需或只需极少冷藏设施的替代样本采集、运输和储存程序，将特别有利于在野外环境中评估排卵活动的研究。
评估使用浸有尿液的试纸替代液体尿液来测定雌酮-3-葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷浓度以评估卵巢活动的可行性。
在整个正常月经周期中每天收集的尿液样本，分别作为液体尿液储存在-20℃，以及作为浸有尿液的试纸，在冰箱中保存3至12个月或在室温下保存1至6个月。通过酶免疫测定法测量这些尿液样本中雌酮-3-葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷的浓度。使用Spearman相关性检验对获得的值进行相关性分析。
在所有使用的储存条件下，使用浸有尿液的试纸估算的雌酮-3葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷浓度模式与液体尿液的模式一致。两种方法获得的值显著相关（p<0.001至0.0001），尽管浸有尿液的试纸给出的值略高。
当需要大量连续样本测量雌酮-3-葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷以评估卵巢活动时，浸有尿液的试纸可用于方便尿液样本的采集、运输和储存。
