使用浸有尿液的试纸而非液体尿液评估卵巢活性的可行性。

Feasibility of using paper impregnated with urine instead of liquid urine for assessing ovarian activity.

作者信息

Wasalathanthri Sudharshani, Tennekoon Kamani H, Sufi S

机构信息

Department of Physiology, Faculty of Medicine, University of Colombo.

出版信息

Ceylon Med J. 2003 Mar;48(1):4-6. doi: 10.4038/cmj.v48i1.3384.

DOI:10.4038/cmj.v48i1.3384

PMID:12795010

Abstract

BACKGROUND

Ovarian activity should ideally be assessed by serial non-invasive methods that require simple procedures for sample collection and storage. Measurement of urinary oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide is a non-invasive method available for assessment of ovarian activity, but transport of large numbers of urine samples is cumbersome and samples need to be stored frozen. An alternative sample collection, transport and storage procedure that is easier to handle and requires no or minimal cold storage facilities will particularly benefit studies in which ovulatory activity needs to be assessed in field settings.

OBJECTIVE

To evaluate the feasibility of using paper impregnated with urine as an alternative to liquid urine for the measurement of oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide concentrations in the assessment of ovarian activity.

METHODS

Urine samples collected daily throughout regular menstrual cycles were stored as liquid urine at -20 degrees C, and as paper impregnated with urine, in the refrigerator for 3 to 12 months or at room temperature for 1 to 6 months. Oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide concentrations were measured in these urine samples by enzyme immunoassay. Values obtained were correlated using Spearman's correlation test.

RESULTS

The pattern of oestrone-3 glucuronide and pregnanediol-3 alpha-glucuronide concentrations estimated using paper impregnated with urine followed that of liquid urine in all storage conditions used. Values obtained by two methods correlated significantly (p < 0.001 to 0.0001) though the paper impregnated with urine gave slightly higher values.

CONCLUSIONS

Paper impregnated with urine can be used to facilitate sample collection, transport and storage of urine when oestrone-3-glucuronide and pregnanediol-3 alpha-glucuronide measurements are required in a large number of serial samples to assess ovarian activity.

摘要

背景

理想情况下，卵巢活动应通过一系列非侵入性方法进行评估，这些方法需要简单的样本采集和储存程序。尿雌酮-3-葡萄糖醛酸苷和孕二醇-3α-葡萄糖醛酸苷的测定是一种可用于评估卵巢活动的非侵入性方法，但大量尿液样本的运输很麻烦，且样本需要冷冻保存。一种更易于操作且无需或只需极少冷藏设施的替代样本采集、运输和储存程序，将特别有利于在野外环境中评估排卵活动的研究。