Gervais Patrick, Martínez de Marañon Iñigo, Evrard Christine, Ferret Eric, Moundanga Sylvie
Laboratoire de Génie des Procédés Alimentaires et Biotechnologiques, ENSBANA, 21000 Dijon, France.
J Biotechnol. 2003 May 8;102(3):269-79. doi: 10.1016/s0168-1656(03)00031-2.
The effect of a rapid temperature increase on the volume of different types of cells was investigated. Experiments were carried out using continuous microscopic image analysis. Volume variation of yeast cells, yeast spheroplasts and human leukaemia cells was measured during the transient phase after a thermal shift. The thermal shift was found to induce rapid increase in cell volume for cells lacking a cell wall (yeast spheroplasts and human leukaemia cells). This increase in cell volume is assumed to be a main cause of the heat shock-induced cell death. A theoretical mechanistic model that explains the behaviour of these cells is finally proposed.
研究了快速升温对不同类型细胞体积的影响。实验采用连续显微镜图像分析进行。在热转移后的瞬态阶段，测量了酵母细胞、酵母原生质体和人白血病细胞的体积变化。发现热转移会导致缺乏细胞壁的细胞（酵母原生质体和人白血病细胞）的细胞体积迅速增加。细胞体积的这种增加被认为是热休克诱导细胞死亡的主要原因。最后提出了一个解释这些细胞行为的理论机制模型。
