酵母原生质体的融合
Fusion of yeast spheroplasts.
作者信息
van Solingen P, van der Plaat J B
出版信息
J Bacteriol. 1977 May;130(2):946-7. doi: 10.1128/jb.130.2.946-947.1977.
Abstract
Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.
摘要
借助聚乙二醇和钙离子，将酿酒酵母两种不同的营养缺陷型菌株（均为a交配型）的原生质球进行融合。在固体培养基中回复为营养细胞后，所产生的合子显示为aa交配型的二倍体细胞。
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本文引用的文献
1
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Nature. 1961 Nov 11;192:580-1. doi: 10.1038/192580a0.
2
Regeneration of yeast protoplasts in agar gels.酵母原生质体在琼脂凝胶中的再生
Exp Cell Res. 1966 Nov-Dec;44(2):640-2. doi: 10.1016/0014-4827(66)90473-3.
3
Purification and properties of an enzyme, zymolyase, which lyses viable yeast cells.一种可裂解活酵母细胞的酶——酵母溶壁酶的纯化及性质
Arch Biochem Biophys. 1972 Nov;153(1):403-6. doi: 10.1016/0003-9861(72)90461-4.
4
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Nature. 1975 May 1;255(5503):66-7. doi: 10.1038/255066a0.
5
Fusion of bacterial protoplasts.细菌原生质体融合
Proc Natl Acad Sci U S A. 1976 Jun;73(6):2151-5. doi: 10.1073/pnas.73.6.2151.
6
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Proc Natl Acad Sci U S A. 1976 Jun;73(6):2147-50. doi: 10.1073/pnas.73.6.2147.
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