van Solingen P, van der Plaat J B
J Bacteriol. 1977 May;130(2):946-7. doi: 10.1128/jb.130.2.946-947.1977.
Spheroplasts of two different auxotrophic strains of Saccharomyces cerevisiae, both of mating type a, were fused with the aid of polyethylene glycol and calcium ions. After reversion to vegetative cells in solid media, the resulting zygotes were shown to be diploid cells of mating type aa.
借助聚乙二醇和钙离子，将酿酒酵母两种不同的营养缺陷型菌株（均为a交配型）的原生质球进行融合。在固体培养基中回复为营养细胞后，所产生的合子显示为aa交配型的二倍体细胞。
