Kamenetzky Laura, Gutierrez Ariana M, Canova Sergio G, Haag Karen L, Guarnera Eduardo A, Parra Alberto, García Gustavo E, Rosenzvit Mara C
Instituto Nacional de Microbiología, ANLIS Dr. Carlos G. Malbrán, Av. Velez Sarsfield 563, CP 1281, Buenos Aires, Argentina.
Infect Genet Evol. 2002 Dec;2(2):129-36. doi: 10.1016/s1567-1348(02)00131-4.
Mitochondrial cytochrome oxidase subunit 1 (CO1) sequencing, Southern blot of a repetitive DNA element and single strand conformation polymorphism of the 5' non-transcribed region of the cytosolic malate dehydrogenase (MDH) gene were used to determine the extent and distribution of Echinococcus granulosus genetic variation in Argentina. Five distinct strains of E. granulosus were shown to exist in the country. The common sheep, Tasmanian sheep, cattle and camel strains were identified in humans. Unlike the situation found in other countries, where the common sheep strain is the major source of human contamination, the Tasmanian sheep and camel strains produced a significant number of human infections in some regions of Argentina. This is the first report of cattle strain in humans in South America. Goats could be the natural intermediate host of the camel strain, which was not identified in humans from other regions so far. More than one genotype was identified in the same geographic area. These findings may have important consequences for human health and the control of hydatid disease. Within-strain differences were also observed, showing the potential of variation of E. granulosus.
利用线粒体细胞色素氧化酶亚基1（CO1）测序、重复DNA元件的Southern印迹分析以及胞质苹果酸脱氢酶（MDH）基因5'非转录区的单链构象多态性，来确定阿根廷细粒棘球绦虫遗传变异的程度和分布。结果表明该国存在5种不同的细粒棘球绦虫菌株。在人类中鉴定出了常见绵羊株、塔斯马尼亚绵羊株、牛株和骆驼株。与其他国家不同，在其他国家常见绵羊株是人类感染的主要来源，而在阿根廷的一些地区，塔斯马尼亚绵羊株和骆驼株导致了大量人类感染。这是南美洲首次关于人类感染牛株的报告。山羊可能是骆驼株的天然中间宿主，目前在其他地区的人类中尚未发现该菌株。在同一地理区域内鉴定出了不止一种基因型。这些发现可能对人类健康和包虫病的控制产生重要影响。同时也观察到了菌株内的差异，显示出细粒棘球绦虫的变异潜力。
