Zhang L H, Chai J J, Jiao W, Osman Y, McManus D P
Molecular Parasitology Unit, Australian Centre for International and Tropical Health and Nutrition, Queensland Institute of Medical Research, Brisbane, Australia.
Parasitology. 1998 Jan;116 ( Pt 1):29-33. doi: 10.1017/s0031182097001881.
Twenty-eight isolates of E. granulosus, collected from humans at surgery, and a range of intermediate hosts, including sheep, cattle and camels from abattoirs in North and South Xinjiang Uygur Autonomous Region, People's Republic of China, were analysed for DNA sequence variation within regions of the mitochondrial cytochrome c oxidase I (COI) and NADH dehydrogenase subunit I (NDI) genes. The isolates were categorized into 2 distinct and uniform genotypic groupings, based on the sequences obtained, and the data clearly indicated that the camel/dog strain (G6 genotype) of E. granulosus as well as the cosmopolitan, common sheep strain (G1 genotype) occur in north Xinjiang. The presence of the camel strain has thus been confirmed in Xinjiang but it is evident from this and a previous molecular genetic survey of E. granulosus isolates from north-western China that the common sheep strain is the most predominant in the region. From the public health perspective, the majority of infected livestock will act as reservoirs of human infection there. During the course of the study, a polymerase chain reaction (PCR)-restriction fragment length polymorphism (RFLP) assay, based on the NDI sequence variation, was developed that allows rapid discrimination of the G1 and G6 genotypes.
从中国新疆维吾尔自治区南北部屠宰场的绵羊、牛和骆驼等一系列中间宿主以及手术患者身上采集了28株细粒棘球绦虫分离株，对其线粒体细胞色素c氧化酶I（COI）和NADH脱氢酶亚基I（NDI）基因区域内的DNA序列变异进行了分析。根据获得的序列，这些分离株被分为2个不同且一致的基因型分组，数据清楚地表明细粒棘球绦虫的骆驼/犬株（G6基因型）以及世界性的普通绵羊株（G1基因型）存在于新疆北部。因此，新疆已证实存在骆驼株，但从本次研究以及之前对中国西北部细粒棘球绦虫分离株的分子遗传学调查可以明显看出，普通绵羊株是该地区最主要的菌株。从公共卫生角度来看，大多数受感染的家畜将成为当地人类感染的储存宿主。在研究过程中，基于NDI序列变异开发了一种聚合酶链反应（PCR）-限制性片段长度多态性（RFLP）检测方法，该方法能够快速区分G1和G6基因型。
