Takamoto Satoshi, Sakura Nobuo, Yashiki Mikio, Kojima Tohru
Department of Pediatrics, Hiroshima University Graduate School of Biomedical Sciences, 1-2-3 Kasumi, Minami-Ku, Hiroshima 734-8551, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2003 Jul 5;791(1-2):365-9. doi: 10.1016/s1570-0232(03)00259-9.
Acrolein, the metabolite of cyclophosphamide and ifosphamide, is an irritant of mucous membranes and seems to play an important role in hemorrhagic cystitis. Several methods are available to reduce the risk of hemorrhagic cystitis. Mesna is a regional detoxificant which inactivates acrolein. However, the interaction of mesna and acrolein has never been reported because no available method can detect acrolein. In this study, we measured acrolein to evaluate the effect of mesna in urine or phosphate-buffered saline using a headspace-solid-phase microextraction gas chromatography and mass spectrometry method which we had previously established. We also investigated the effect of mesna at different conditions of pH. Mesna was effective in a dose-dependent (10 microM to 20 mM) fashion in both urine and phosphate-buffered saline and completely inactivated acrolein at concentrations over 10 mM. Furthermore, mesna was more effective in alkaline conditions than in acid.
丙烯醛是环磷酰胺和异环磷酰胺的代谢产物，是一种黏膜刺激物，似乎在出血性膀胱炎中起重要作用。有几种方法可降低出血性膀胱炎的风险。美司钠是一种局部解毒剂，可使丙烯醛失活。然而，由于没有可用的方法能检测丙烯醛，美司钠与丙烯醛的相互作用从未被报道过。在本研究中，我们使用先前建立的顶空-固相微萃取气相色谱和质谱法测量丙烯醛，以评估美司钠在尿液或磷酸盐缓冲盐水中的效果。我们还研究了不同pH条件下美司钠的效果。美司钠在尿液和磷酸盐缓冲盐水中均呈剂量依赖性（10微摩尔至20毫摩尔）有效，且在浓度超过10毫摩尔时能完全使丙烯醛失活。此外，美司钠在碱性条件下比在酸性条件下更有效。
