用弓形虫热休克蛋白70、热休克蛋白30和表面抗原1基因进行DNA疫苗接种诱导保护性免疫
Induction of protective immunity by DNA vaccination with Toxoplasma gondii HSP70, HSP30 and SAG1 genes.
作者信息
Mohamed Rabie M, Aosai Fumie, Chen Mei, Mun Hye-Seong, Norose Kazumi, Belal Usama S, Piao Lian-Xun, Yano Akihiko
机构信息
Department of Infection & Host Defense, Graduate School of Medicine, Chiba University, 1-8-1 Inohana, Chuo-Ku, Chiba 260-8670, Japan.
出版信息
Vaccine. 2003 Jun 20;21(21-22):2852-61. doi: 10.1016/s0264-410x(03)00157-9.
The vaccination with Toxoplasma gondii heat shock protein 70 (T.g.HSP70) gene (a virulent tachyzoite-specific) induced the most prominent reduction in T. gondii loads in various organs of B6 and BALB/c mice at the acute and chronic phases of toxoplasmosis compared with T.g.HSP30 (a bradyzoite-specific) and SAG1 (a tachyzoite-specific) genes. A single gene gun vaccination with 2 microg of T.g.HSP70 gene induced a significant reduction in the number of T. gondii organisms compared with 50 microg of T.g.HSP70 gene vaccination by intramuscular (i.m.) or intraperitoneal (i.p.) injection. The vaccine effects of T.g.HSP70 gene persisted for more than 3 months.
与弓形虫热休克蛋白30基因（一种缓殖子特异性基因）和SAG1基因（一种速殖子特异性基因）相比，接种弓形虫热休克蛋白70（T.g.HSP70）基因（一种强毒速殖子特异性基因）在弓形虫病的急性和慢性阶段能使B6和BALB/c小鼠各器官中的弓形虫负荷显著降低。与通过肌肉注射（i.m.）或腹腔注射（i.p.）接种50微克T.g.HSP70基因相比，单次基因枪接种2微克T.g.HSP70基因能使弓形虫生物体数量显著减少。T.g.HSP70基因的疫苗效果持续超过3个月。
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