Mun H S, Aosai F, Yano A
Department of Parasitology, Chiba University School of Medicine, Japan.
Microbiol Immunol. 1999;43(5):471-9. doi: 10.1111/j.1348-0421.1999.tb02430.x.
Production of antibodies against Toxoplasma gondii (T. gondii)-derived stress proteins, T. gondii HSP70 (T.g.HSP70) and T.g.HSP30/bagl, in C57BL/6 and BALB/c mice perorally infected with cysts of the avirulent Fukaya strain of T. gondii was analyzed. Production of anti-T.g.HSP70 IgG antibodies was transient, whereas production of anti-T.g.HSP30/bag1 IgG antibodies persisted after infection in both C57BL/6 and BALB/c mice. C57BL/6 mice, a susceptible strain, predominantly produced IgG antibodies specific for T.g.HSP70, whereas BALB/c mice, a resistant strain, predominantly produced IgG antibodies specific for T.g.HSP30/bag1, after T. gondii infection. Immunization with rT.g.HSP30/bag1 enhanced, whereas immunization with rT.g.HSP70 reduced host protective immunity against T. gondii infection with a cyst-forming avirulent strain, Fukaya, and a virulent strain, RH.
分析了口服感染无毒力的弓形虫深谷株包囊的C57BL/6和BALB/c小鼠中抗弓形虫（Toxoplasma gondii，T. gondii）衍生应激蛋白、弓形虫热休克蛋白70（T.g.HSP70）和T.g.HSP30/bag1抗体的产生情况。抗T.g.HSP70 IgG抗体的产生是短暂的，而在C57BL/6和BALB/c小鼠感染后，抗T.g.HSP30/bag1 IgG抗体的产生持续存在。在弓形虫感染后，易感品系C57BL/6小鼠主要产生针对T.g.HSP70的特异性IgG抗体，而抗性品系BALB/c小鼠主要产生针对T.g.HSP30/bag1的特异性IgG抗体。用重组T.g.HSP30/bag1免疫可增强宿主对形成包囊的无毒力菌株深谷株和有毒力菌株RH的弓形虫感染的保护性免疫，而用重组T.g.HSP70免疫则降低这种保护性免疫。
