Mukhopadhaya A, Mahalanabis D, Khanam J, Chakrabarti M K
Division of Pathophysiology, National Institute of Cholera & Enteric Diseases, P-33, C.I.T. Road, Scheme-XM, Beliaghata, Kolkata 700010, West Bengal, India.
Vaccine. 2003 Jun 20;21(21-22):3043-50. doi: 10.1016/s0264-410x(03)00111-7.
Oral immunization of rabbits with four doses of 10(11) heat-killed Shigella flexneri 2a showed 100% protection against challenge with virulent S. flexneri 2a. After orally immunizing Guinea pigs with four doses of heat-killed S. flexneri 2a 100% protection could be shown against ocular challenge with the same virulent S. flexneri 2a strain but this conferred no protection against challenge with Shigella dysenteriae type 1. In enzyme-linked immunosorbent assay (ELISA) and immunoblot experiments both whole cell lysate-envelope (WCL-E) fraction and outer membrane proteins (OMPs) were recognized by the antisera. Though protective mechanism in shigellosis is not established with certainty, outer membrane proteins (specially 38, 34, 23 and 20kDa proteins) may be the major antigens in the induction of protective immune responses as indicated by this observation.
用四剂10(11) 热灭活的福氏志贺菌2a对兔子进行口服免疫，结果显示对强毒力福氏志贺菌2a的攻击具有100% 的保护作用。用四剂热灭活的福氏志贺菌2a对豚鼠进行口服免疫后，对相同强毒力福氏志贺菌2a菌株的眼部攻击可显示100% 的保护作用，但这对痢疾志贺菌1型的攻击没有保护作用。在酶联免疫吸附测定 (ELISA) 和免疫印迹实验中，全细胞裂解物-包膜 (WCL-E) 组分和外膜蛋白 (OMPs) 均能被抗血清识别。虽然志贺菌病的保护机制尚未完全确定，但如本观察所示，外膜蛋白（特别是38、34、23和20kDa蛋白）可能是诱导保护性免疫反应的主要抗原。
