Bell Richard L, Rodd-Henricks Zachary A, Kuc Kelly A, Lumeng Lawrence, Li Ting Kai, Murphy James M, McBride William J
Department of Psychiatry, Indiana University School of Medicine, Indianapolis 46202, USA.
Alcohol. 2003 Apr;29(3):137-48. doi: 10.1016/s0741-8329(03)00022-3.
The objectives of this study were to assess the effects of access to different concentrations of ethanol and sex of the animal on ethanol consumption during periadolescence [postnatal days (PNDs) 30-60] in alcohol-preferring (P) rats. On PND 28, female and male P pups were single housed in hanging stainless steel cages with ad libitum access to water and food. Beginning on PND 30, the rats were also given access to either a single concentration [15% volume/volume (vol./vol.)] or multiple concentrations [10%, 20%, and 30% (vol./vol.)] of ethanol. Differences between sex (male vs. female) and ethanol conditions (single concentration vs. multiple concentrations), for the average amount of ethanol consumed for each week (starting on PND 33) of access, were examined. Analyses of the data for ethanol drinking revealed significant (P<.025) main effects of week and ethanol condition, as well as a significant weekxethanol condition interaction. For the first week, both male and female P pups consumed more ethanol under the multiple-ethanol-concentration condition than under the single-ethanol-concentration condition. However, across the second through fourth weeks, this pattern was seen only in female P pups. When preference for one concentration of ethanol over the other concentrations was assessed, it was found that male P pups tended to choose the 30% concentration over the 10% and 20% concentrations, whereas female P pups did not display a preference. The findings of this study corroborate previous work indicating that periadolescent P rats readily acquire high-ethanol-drinking behavior and that, similar to adult P rats, concurrent access to multiple concentrations of ethanol further enhances ethanol intake. These findings suggest to us that innate genetically influenced mechanisms promoting high ethanol intake are present at this stage of development.
本研究的目的是评估在围青春期（出生后第30 - 60天）接触不同浓度乙醇以及动物性别对嗜酒（P）大鼠乙醇消耗量的影响。在出生后第28天，将雌性和雄性P幼鼠单独饲养在悬挂的不锈钢笼中，自由获取水和食物。从出生后第30天开始，大鼠还可以接触单一浓度[15%体积/体积（vol./vol.）]或多种浓度[10%、20%和30%（vol./vol.）]的乙醇。研究了性别（雄性与雌性）和乙醇条件（单一浓度与多种浓度）之间，在开始接触乙醇后每周（从出生后第33天开始）乙醇平均消耗量的差异。对乙醇饮用数据的分析显示，周和乙醇条件存在显著（P<0.025）的主效应，以及显著的周×乙醇条件交互作用。在第一周，雄性和雌性P幼鼠在多种乙醇浓度条件下比在单一乙醇浓度条件下消耗更多的乙醇。然而，在第二周至第四周，这种模式仅在雌性P幼鼠中出现。当评估对一种乙醇浓度相对于其他浓度的偏好时，发现雄性P幼鼠倾向于选择30%浓度而非10%和20%浓度，而雌性P幼鼠没有表现出偏好。本研究结果证实了先前的研究工作，表明围青春期的P大鼠容易养成高乙醇饮用行为，并且与成年P大鼠类似，同时接触多种浓度的乙醇会进一步增加乙醇摄入量。这些发现向我们表明，在这个发育阶段存在促进高乙醇摄入量的先天遗传影响机制。
