Bell Richard L, Rodd Zachary A, Hsu Cathleen C, Lumeng Lawrence, Li Ting-Kai, Murphy James M, McBride William J
Department of Psychiatry, Institute of Psychiatric Research, Indiana University School of Medicine, Indianapolis, IN 46202, USA.
Alcohol. 2004 Jun;33(2):107-15. doi: 10.1016/j.alcohol.2004.06.001.
The objectives of the current study were to assess the effects of access to different concentrations of ethanol and sex of the animal on ethanol consumption of high-alcohol-drinking (HAD-1 and HAD-2) rats during adolescence [postnatal days (PNDs) 30 through 60]. At the beginning of adolescence (PND 30), the rats were given concurrent access to either a single concentration [15% volume/volume (vol./vol.)] or multiple concentrations [10%, 20%, and 30% (vol./vol.)] of ethanol and water. Analyses of ethanol consumption data revealed significant (P < .025) main effects of line, ethanol condition, and week, and a significant line by sex by ethanol condition by week interaction. For the first week, both male and female HAD-1 and HAD-2 rats consumed more ethanol under the multiple ethanol concentration condition than under the single ethanol concentration condition. However, across the second through fourth weeks, this pattern was seen primarily in male and female HAD-1 rats and to a lesser degree in female HAD-2 rats. In general, female rats consumed more fluids than consumed by male rats, and male rats displayed a higher preference for ethanol over water ratio than observed for their female counterparts. In addition, in comparison with HAD-2 rats, HAD-1 rats drank more ethanol and displayed a higher preference for ethanol ratio. Overall, the current study results indicate that, compared with access to a single concentration (which is used in most studies), concurrent access to multiple concentrations of ethanol produced significantly higher ethanol intakes in periadolescent HAD rats, supporting the suggestion that this ethanol drinking condition would have a greater impact on neuronal development. In addition, although the replicate lines were selectively bred by using the same criteria and foundation stock, the higher ethanol intakes of the HAD-1 line, compared with intakes for the HAD-2 line, seen in the current study support the suggestion that there are some differences in their genetic make-up, affecting ethanol intake, which are expressed during periadolescence.
本研究的目的是评估在青春期（出生后第30天至60天），给予不同浓度乙醇以及动物性别对高饮酒量（HAD - 1和HAD - 2）大鼠乙醇摄入量的影响。在青春期开始时（出生后第30天），给予大鼠同时接触单一浓度[15%体积/体积（vol./vol.）]或多种浓度[10%、20%和30%（vol./vol.）]的乙醇和水。对乙醇摄入量数据的分析显示，品系、乙醇条件和周数存在显著（P < 0.025）的主效应，以及品系×性别×乙醇条件×周数的显著交互作用。在第一周，雄性和雌性的HAD - 1和HAD - 2大鼠在多种乙醇浓度条件下比在单一乙醇浓度条件下消耗更多的乙醇。然而，在第二周至第四周期间，这种模式主要见于雄性和雌性HAD - 1大鼠，而在雌性HAD - 2大鼠中程度较轻。一般来说，雌性大鼠比雄性大鼠消耗更多的液体，并且雄性大鼠对乙醇与水比例的偏好高于雌性大鼠。此外，与HAD - 2大鼠相比，HAD - 1大鼠饮用更多的乙醇并且对乙醇比例表现出更高的偏好。总体而言，本研究结果表明，与大多数研究中使用的单一浓度接触相比，同时接触多种浓度的乙醇在青春期前的HAD大鼠中产生了显著更高的乙醇摄入量，支持了这种乙醇饮用条件对神经元发育有更大影响的观点。此外，尽管这些重复品系是使用相同的标准和基础种群进行选择性培育的，但在本研究中观察到的HAD - 1品系与HAD - 2品系相比更高的乙醇摄入量支持了这样的观点，即它们的基因组成存在一些差异，影响乙醇摄入量，这些差异在青春期前表现出来。
