Varkarakis M J, Papahadjopoulos D, Murphy G P
Proc Eur Dial Transplant Assoc. 1975;11:294-9.
After 24-hr storage of canine kidneys with extracellular or intracellular (Ursol) solutions, the cortical and medullary renal ATPase enzymes (total Na+ + K+ and Mg-ATPase, Na+ + K+ -ATPase, and Mg-ATPase) were examined. It was found that storage with extracellular solution decreased all cortical enzymes. This was not the case with intracellular solution or in kidneys cooled and stored without any solution. A decrease in the potassium concentration of the Ursol solution decreased cortical (Na+ + K+)-ATPase enzymatic activity. The medullary enzymatic changes were similar in the different groups, and lower than in unstored controls. It appears that the changes on the level of the ATPase enzyme system which is related to the cation transport system might play a significant role in explaining the different results seen in clinical or experimental renal preservation systems. These changes can be related to the injury of preservation due to environmental effects of the cation concentrations and to a lesser degree to the damage of the enzyme system which provides the energy for cation transport.
用细胞外或细胞内（熊果酚）溶液对犬肾进行24小时保存后，检测了肾皮质和髓质的ATP酶（总钠钾和镁ATP酶、钠钾ATP酶和镁ATP酶）。发现用细胞外溶液保存会降低所有皮质酶的活性。细胞内溶液保存的情况或肾脏在无溶液状态下冷却保存的情况则并非如此。熊果酚溶液中钾浓度的降低会降低皮质（钠钾）ATP酶的活性。不同组髓质酶的变化相似，且低于未保存的对照组。与阳离子转运系统相关的ATP酶系统水平的变化似乎在解释临床或实验性肾脏保存系统中所见的不同结果方面可能起重要作用。这些变化可能与阳离子浓度的环境效应导致的保存损伤有关，在较小程度上也与为阳离子转运提供能量的酶系统损伤有关。
