Ramella S G, Hadj-Aïssa A, Barbieux A, Steghens J P, Colpart J J, Zech P, Pozet N
Department of Renal Functional Exploration, Edouard Herriot Hospital, Lyon, France.
Nephrol Dial Transplant. 1995;10(6):842-6.
The isolated perfused rat kidney (IPK) model was used to assess initial renal function after 24 h preservation in 3 different cold storage solutions: EuroCollins (EC), a solution prepared according to the formulation of Belzer's solution (High-K+ UW) and a high Na(+)-low K+ Belzer UW solution (High-Na+ UW). GFR and FRNa were measured after 24 h cold storage in each of the solutions during 60 min, and were compared to values obtained in a control group in which renal function was measured immediately after the kidneys had been harvested. ATP and CP were measured in fresh renal tissue, in kidneys preserved for 24 h in each solution, in control IPK, and in reperfused IPK after they had been preserved for 24 h. Main results showed that preservation in either solution caused a dramatic decrease in GFR and in FRNa within the first 60 min following reperfusion of cold-stored kidneys. However FRNa was significantly higher in the High-Na+ UW group. ATP and CP content were decreased to approximately 10% of basal values in all experimental groups after cold-storage. Normothermic reperfusion of IPK after cold-storage induced a restoration of ATP levels, but CP content decreased further. There was no significant difference in ATP and CP content between cold-storage solutions, nor any correlation between metabolic and functional parameters.
采用离体灌注大鼠肾（IPK）模型，评估在3种不同冷藏溶液中保存24小时后的初始肾功能：EuroCollins（EC）、根据Belzer溶液配方配制的溶液（高钾UW）和高钠低钾Belzer UW溶液（高钠UW）。在每种溶液中冷藏24小时后，于60分钟内测量肾小球滤过率（GFR）和钠排泄分数（FRNa），并与在肾脏收获后立即测量肾功能的对照组所获数值进行比较。在新鲜肾组织、在每种溶液中保存24小时的肾脏、对照IPK以及在保存24小时后再灌注的IPK中测量三磷酸腺苷（ATP）和磷酸肌酸（CP）。主要结果显示，在冷藏肾脏再灌注后的最初60分钟内，保存在任何一种溶液中均导致GFR和FRNa显著下降。然而，高钠UW组的FRNa显著更高。冷藏后，所有实验组的ATP和CP含量均降至基础值的约10%。冷藏后IPK的常温再灌注诱导ATP水平恢复，但CP含量进一步下降。冷藏溶液之间的ATP和CP含量无显著差异，代谢和功能参数之间也无任何相关性。
