一种对枯草芽孢杆菌孢子有效的杀孢子试剂。

An effective sporicidal reagent against Bacillus subtilis spores.

作者信息

Kida Nori, Mochizuki Yasushi, Taguchi Fumiaki

机构信息

Niigata Quarantine Station, Ministry of Health, Labour and Welfare, 1-5-4, Ryugashima, Niigata, Niigata 950-0072, Japan.

出版信息

Microbiol Immunol. 2003;47(4):279-83. doi: 10.1111/j.1348-0421.2003.tb03396.x.

DOI:10.1111/j.1348-0421.2003.tb03396.x

PMID:12801065

Abstract

We developed a reagent which showed significant sporicidal activity against Bacillus subtilis spores. This reagent was composed of ethylenediaminetetraacetic acid, disodium salt (EDTA-2Na), ferric chloride hexahydrate (FeCl3 x 6H2O) and ethanol (tentatively designated as the ethanol reagent). The ethanol reagent showed pH- and temperature-dependent sporicidal activity. At pH 0.3, its activity was almost the same as that of 0.05% sodium hypochlorite at 20 C and was higher at 37 C than at 20 C. The activity of the ethanol reagent was similar both with and without 10% serum. The ethanol reagent might be applicable for disinfecting Bacillus spores.

摘要

我们研发了一种对枯草芽孢杆菌孢子具有显著杀孢子活性的试剂。该试剂由乙二胺四乙酸二钠盐（EDTA - 2Na）、六水合氯化铁（FeCl₃·6H₂O）和乙醇组成（暂定为乙醇试剂）。乙醇试剂的杀孢子活性呈现出pH值和温度依赖性。在pH 0.3时，其活性与20℃下0.05%次氯酸钠的活性几乎相同，且在37℃时比在20℃时更高。有无10%血清时乙醇试剂的活性相似。乙醇试剂可能适用于枯草芽孢杆菌孢子的消毒。