脑一氧化氮合酶是一种血红素蛋白。
Brain nitric oxide synthase is a haemoprotein.
作者信息
Klatt P, Schmidt K, Mayer B
机构信息
Institut für Pharmacokologie und Toxikologie, Karl-Franzens-Universität Graz, Austria.
出版信息
Biochem J. 1992 Nov 15;288 ( Pt 1)(Pt 1):15-7. doi: 10.1042/bj2880015.
Abstract
Brain nitric oxide (NO) synthase showed pyridine haemochrome spectra typical of ferroprotoporphyrin IX-containing enzymes. The haem content of purified NO synthase was in the range 0.7-0.9 mol/mol of 160 kDa subunit. In the presence of CO, NO, KCN and miconazole, the L-citrulline-forming activity of NO synthase was markedly diminished, demonstrating that enzyme-bound haem is involved in enzymic NO synthesis.
摘要
脑一氧化氮（NO）合酶呈现出含亚铁原卟啉IX的酶所特有的吡啶血红素光谱。纯化的NO合酶的血红素含量在每摩尔160 kDa亚基含0.7 - 0.9摩尔的范围内。在一氧化碳、一氧化氮、氰化钾和咪康唑存在的情况下，NO合酶的生成L - 瓜氨酸的活性显著降低，表明酶结合的血红素参与了酶促NO合成。
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