无需DNA纯化，直接对通过聚合酶链反应从细菌培养物中扩增出的16S rDNA进行自动测序。
Direct automated sequencing of 16S rDNA amplified by polymerase chain reaction from bacterial cultures without DNA purification.
作者信息
Hiraishi A
机构信息
Laboratory of Environmental Biotechnology, Konishi Co., Ltd, Tokyo, Japan.
出版信息
Lett Appl Microbiol. 1992 Nov;15(5):210-3. doi: 10.1111/j.1472-765x.1992.tb00765.x.
Abstract
The 16S rRNA gene from various bacterial cultures was amplified by the polymerase chain reaction without DNA purification, and sequenced directly by using a laser fluorescent DNA sequencer and Tth polymerase with a cycle sequencing protocol. The described procedures provide almost complete 16S rDNA sequence data within a couple of days and facilitate systematic studies.
摘要
通过聚合酶链反应对来自各种细菌培养物的16S rRNA基因进行扩增，无需进行DNA纯化，然后使用激光荧光DNA测序仪和Tth聚合酶并采用循环测序方案直接进行测序。所描述的程序可在几天内提供几乎完整的16S rDNA序列数据，并有助于进行系统研究。
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