He Yang, Simões Marta Filipa, de la Haba Rafael R, Santos-Pereira Cátia, Sousa Joana, Gomes Joana S, Silvério Sara C, Rodrigues Lígia R, Antunes André
State Key Laboratory of Lunar and Planetary Sciences, Macau University of Science and Technology (MUST), Taipa, Macau SAR, PR China.
China National Space Administration (CNSA), Macau Center for Space Exploration and Science, Taipa, Macau SAR, PR China.
Int J Syst Evol Microbiol. 2025 Jul;75(7). doi: 10.1099/ijsem.0.006840.
A novel moderately halophilic and alkaliphilic Gram-stain-negative, strictly aerobic, bacterial strain (N2) was isolated from an inland saltern in central Portugal. The taxonomic position of this isolate was determined based on polyphasic taxonomic and phylogenomic analysis. Phylogenetic analysis based on 16S rRNA gene sequences indicated that isolate N2 belongs to the genus , showing the highest similarity to 2W32 (98.14%). Phylogenomic analysis based on whole genomes, using the up-to-date bacterial core gene sets (92 genes), showed that strain N2 formed a distinct monophyletic lineage within the genus . The cells of N2 were motile rods that grew at temperatures between 30 and 40 °C (optimum at 35 °C), pH levels of 6.0-11.0 (optimum at pH 9.0) and salinities of 13-20 % (w/v) NaCl (optimum at 15% NaCl). Cells tested positive for oxidase and catalase activity. The predominant isoprenoid quinone was menaquinone-7 (MK-7), and the major fatty acids were iso-C, anteiso-C, C and/or 10-methyl C. The polar lipids included two aminolipids, two glycolipids and seven phospholipids. The DNA G+C content was 42.0 mol%. Based on phylogenetic, phylogenomic, genomic, phenotypic and chemotaxonomic data, we propose that strain N2 (=KCTC 102228=MCCC 1K08942) represents a novel species within the genus , with the name sp. nov. We also propose the reclassification of as sp. nov.
从葡萄牙中部的一个内陆盐场分离出了一株新型的中度嗜盐且嗜碱的革兰氏染色阴性、严格需氧的细菌菌株（N2）。基于多相分类和系统基因组分析确定了该分离株的分类地位。基于16S rRNA基因序列的系统发育分析表明，分离株N2属于 属，与 2W32的相似性最高（98.14%）。使用最新的细菌核心基因组集（92个基因）基于全基因组进行的系统基因组分析表明，菌株N2在该属内形成了一个独特的单系分支。N2的细胞为运动性杆状，在30至40°C（最适温度为35°C）、pH值为6.0 - 11.0（最适pH为9.0）以及盐度为13 - 20%（w/v）NaCl（最适盐度为15% NaCl）的条件下生长。细胞的氧化酶和过氧化氢酶活性检测呈阳性。主要的类异戊二烯醌为甲基萘醌-7（MK-7），主要脂肪酸为异-C、anteiso-C、C 和/或10-甲基-C。极性脂质包括两种氨基脂质、两种糖脂和七种磷脂。DNA G+C含量为42.0 mol%。基于系统发育、系统基因组、基因组、表型和化学分类学数据，我们提议菌株N2（=KCTC 102228 = MCCC 1K08942）代表 属内的一个新物种，命名为 新种。我们还提议将 重新分类为 新种。
