Nishizawa M, Wakabayashi-Ito N, Nagata S
Osaka Bioscience Institute, Japan.
FEBS Lett. 1991 Apr 22;282(1):95-7. doi: 10.1016/0014-5793(91)80452-9.
Granulocyte colony-stimulating factor (G-CSF) is produced from macrophages in response to lipopolysaccharide (LPS). GPE1, a cis-acting element of the G-CSF gene promoter, functioned as an LPS-inducible element. We isolated cDNA and a chromosomal gene encoding the mouse GPE1-binding protein (GPE1-BP). A 150-amino acid protein deduced from the cDNA has a basic domain and a leucine zipper motif, and seems to be identical to that of recently isolated Ig/EBP. The nuclear extract from COS cells transfected with the cDNA showed GPE1-binding activity. Transcripts were ubiquitously detected, and may be spliced from two exons of a single gene.
粒细胞集落刺激因子（G-CSF）由巨噬细胞响应脂多糖（LPS）产生。GPE1是G-CSF基因启动子的顺式作用元件，作为LPS诱导元件发挥作用。我们分离出了编码小鼠GPE1结合蛋白（GPE1-BP）的cDNA和染色体基因。从该cDNA推导的一个150个氨基酸的蛋白质具有一个碱性结构域和一个亮氨酸拉链基序，似乎与最近分离出的Ig/EBP相同。用该cDNA转染的COS细胞的核提取物显示出GPE1结合活性。转录本在各处都能检测到，可能是从单个基因的两个外显子剪接而来。
