热带假丝酵母几株菌株细胞壁甘露聚糖中抗原性低聚甘露糖侧链的分布

Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis.

作者信息

Kobayashi Hidemitsu, Oyamada Hiroko, Matsuda Kyoko, Shibata Nobuyuki, Suzuki Shigeo

机构信息

Department of Microbiology, Kyushu Women's University, 1-1 Jiyugaoka, Yahata-nishi-ku, Kitakyushu, Fukuoka 807-8586, Japan.

出版信息

Arch Microbiol. 2003 Jul;180(1):76-80. doi: 10.1007/s00203-003-0550-7. Epub 2003 Jun 7.

DOI:10.1007/s00203-003-0550-7

PMID:12802478

Abstract

In order to clarify the distribution of antigenic oligomannosyl side chains in the cell wall mannans of the pathogenic yeast Candida tropicalis, the chemical structure of mannans isolated from four C. tropicalis strains was investigated using nuclear magnetic resonance, two-dimensional homonuclear Hartmann-Hahn (2D-HOHAHA) spectroscopy. Two-dimensional maps of the 2D-HOHAHA clearly showed the distribution of oligomannosyl side chains in the mannans. The linear side chain Manalpha1-3Manalpha1-(2Manalpha1-)(n)2Man [n> or =2] is present in the mannans from C. tropicalis IFO 0589 and IFO 1400, but not in the mannans from IFO 0199 and IFO 1647. The mannan of IFO 0589 is the only mannan with the branched side chains, Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)(n)2Man and Manalpha1-2Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)(n)2Man [n> or =2]. However, this mannan lacked the phosphate group and the beta-1,2-linked oligomannosyl side chain which are features of this group. The mannans of the C. tropicalis strains IFO 0589 and IFO 1400 possessed the side chains containing an alpha-1,3-linked mannose residue previously observed in Candida albicans.

摘要

为了阐明致病性热带假丝酵母细胞壁甘露聚糖中抗原性低聚甘露糖侧链的分布情况，利用核磁共振、二维同核Hartmann-Hahn（2D-HOHAHA）光谱对从四株热带假丝酵母菌株中分离得到的甘露聚糖的化学结构进行了研究。2D-HOHAHA的二维图谱清晰地显示了甘露聚糖中低聚甘露糖侧链的分布。线性侧链Manα1-3Manα1-(2Manα1-)(n)2Man [n≥2]存在于热带假丝酵母IFO 0589和IFO 1400的甘露聚糖中，但不存在于IFO 0199和IFO 1647的甘露聚糖中。IFO 0589的甘露聚糖是唯一具有分支侧链Manα1-3[Manα1-6]Manα1-(2Manα1-)(n)2Man和Manα1-2Manα1-3[Manα1-6]Manα1-(2Manα1-)(n)2Man [n≥2]的甘露聚糖。然而，这种甘露聚糖缺乏该类别的特征性磷酸基团和β-1,2-连接的低聚甘露糖侧链。热带假丝酵母菌株IFO 0589和IFO 1400的甘露聚糖具有先前在白色念珠菌中观察到的含有α-1,3-连接甘露糖残基的侧链。